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Description
Mouse TGFb2 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Cell culture supernatant: Centrifuge at 1000×g for 20 minutes, take the supernatant for detection, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Preparation of standard gradient working solution: Add 1mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 2000pg/mL). Then dilute to the following concentrations: 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, and 0pg/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each tube. Pipette 500uL of the 2000pg/mL standard working solution into the first EP tube and mix thoroughly to make a 1000pg/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube is used as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP enzyme conjugate are sequentially added to microwells pre-coated with a Transforming Growth Factor Beta 2 (TGFb2) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of Transforming Growth Factor Beta 2 (TGFb2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Transforming Growth Factor Beta 2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Transforming growth factor β2 (TGF-β2) is a secreted protein known as a cytokine. It has numerous cellular functions and plays an important role in embryonic development (alternative names: glioblastoma-derived T-cell suppressive factor, G-TSF, BSC-1 cytostatic agent, Polyergin, Cetermin). It is an extracellular glycosylated protein known to inhibit the effects of interleukin-dependent T-cell tumors. This protein has two named isoforms, generated by alternative splicing of the same gene (i.e., TGFB2). | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 31.25-2000 pg/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell culture supernatant and other biological fluids |
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4.2 ★★★★★
Based on 17 reviews
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Product Reviews
★★★★★ 5
I'm impressed! Simple but quite effective, affordable, properly designed
Color: Walnut Wood
I normally don't review items unless I am very impressed or feel I need to review to warn others of a particularly poor product.
In this case I am highly impressed! Great price (compare to others made from plastic), well designed and quality construction.
Simple but quite effective. Quality wood support, smooth sanded and beautifully finished.. The wood support is designed and created to properly support headphones without damage to the headband as a simple hook or peg design might
I have a home recording studio and don't want some cheap shlopck headphone holder to damage my $300 headphones in any way.
This support is very reasonably priced, and offers 2 ways to mount. Included double sided self adhesive or using a screw through the hole at the top of the metal bracket. Screw mounting hardware is included.
The metal bar used is incredibly robust but is well suited aesthetically to complement the wood rest.
I would recommend to my friends or anyone who wants a quality, I expensive, and affection headphones holder. Don't hesitate to purchase, you won't be sorry!
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Reviewed in the United States on April 5, 2025
★★★★★ 5
Awesome Quality great for my headphones
Color: Walnut Wood
I recently purchased the Wood Headphone & Headset Wall Mount in Black, and it has truly transformed my workspace! The space-saving design is a game-changer, instantly decluttering my desk and giving it a sleek, organized look.
The curved bearing area is a thoughtful touch, ensuring that my headphone head beam remains in perfect condition without any risk of deformation or dents. The combination of aluminum alloy and wood not only gives it a modern aesthetic but also makes it incredibly sturdy and strong. I was initially skeptical about using double-sided tape, but the 3M tape provided proved to be exceptionally reliable. The mount is securely adhered to my wall, and I can trust that it will never break or fall.
What I love most is its versatility – it accommodates all types of headphones, even the heaviest ones, and I've even used it to hang my handbags and backpacks. It's truly a versatile and practical solution for anyone looking to optimize their workspace. I highly recommend the Wood Headphone & Headset Wall Mount for its durability, functionality, and the aesthetic upgrade it brings to any room!
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Reviewed in the United States on December 3, 2023
★★★★★ 5
Good product
Color: Walnut Wood
Good product. Looks good, attaches firmly to a table with the 3m tape, holds my headphones very nicely.
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Reviewed in the United States on April 9, 2026
★★★★★ 5
Elegant, simple and effective
Color: Walnut Wood
Nothing negative to say about this holder. Does what it should while not taking up much space. The wood grain looks great. Definitely recommended.
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Reviewed in the United States on January 20, 2026
★★★★★ 4
I mounted it with a drawer pull
Color: Walnut Wood, Color: Walnut Wood
Functionality: 5 , Design : 5 , Price: 3
I was looking for a way to store my headphones and not clutter my desk. I also wanted to make sure the padding doesn’t get deformed by hanging them on a hook.
This wall mounted option met all my requirements, even though I feel it’s a bit pricey for just a small piece of wood and metal.
It’s nicely finished and the shape will minimize leaving a dent in the padding of the headphones. I was worried that the color wouldn’t match my desk but luckily it was very close.
Based on other reviews I didn’t trust the adhesive. Instead of drilling a hole into my desk I thought I would try attaching it with one of the drawer pulls. The hole of the wall hanger was just a millimeter too small for the screw of the drawer pull, but a quick mod with my power drill and it fits perfectly.
I’m glad I didn’t drill into my desk, and if the position ends up inconvenient I can easily swap it to a different drawer.
I would highly recommend this product.
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Reviewed in the United States on July 21, 2023