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Description
Biotin Mouse Anti-Rat CD25 Antibody (S-R676)Product Specification Host Mouse Antigen CD25 Synonyms Peripheral myelin protein 22; PMP 22; Protein CD25; SR13 myelin protein; Schwann cell membrane glycoprotein (SAG); Cd25; Pmp22 Location Cell membrane Accession P25094 Clone Number S R676 Antibody Type Mouse mAb Isotype IgG1,k Application FCM Reactivity Rt Positive Sample SD Rat splenocytes Purification Protein G Concentration 0. 2 mg ml Conjugation Biotin Physical Appearance Liquid Storage Buffer
Product Specification
| Host | Mouse |
| Antigen | CD25 |
| Synonyms | Peripheral myelin protein 22; PMP-22; Protein CD25; SR13 myelin protein; Schwann cell membrane glycoprotein (SAG); Cd25; Pmp22 |
| Location | Cell membrane |
| Accession | P25094 |
| Clone Number | S-R676 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Rt |
| Positive Sample | SD Rat splenocytes |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Rt |
Background
CD25, also known as the interleukin-2 receptor alpha chain (IL-2Rα), is a 55 kDa type I transmembrane glycoprotein essential for immune regulation, forming part of the high-affinity IL-2 receptor complex (with IL-2Rβ and the common γ chain) that mediates IL-2 signaling in activated T cells, B cells, NK cells, and particularly in CD4+ regulatory T cells (Tregs), where its expression is critical for maintaining immune homeostasis and self-tolerance, and is dynamically upregulated upon T cell activation through transcriptional control involving NF-AT factors, while also existing in a soluble form (sCD25) that can modulate IL-2 availability and serve as a clinical biomarker in autoimmune diseases, infections, and cancers.
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4.6 ★★★★★
Based on 13 reviews
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Product Reviews
★★★★★ 4
Quirky, small, and not for everybody
Color: Black/White/Black, Color: Black/White/Black
This is an attractive little watch, similar to the older Timex Expedition, but changes have been made.
Like its earlier version, this is a small, light timepiece. The band is something different, with the case being held in place by an elastic insert sewn in. Pro - This watch stays put on the wrist, making it a good work watch. Con - pins will have to come out to remove band or change the battery. Another unique feature is the use of triangle slot retention screws on the back of the case, no doubt requiring a special screwdriver most folks probably don't have on hand. The band Velcro's into place, appears best for small or medium wrists, and stays where one puts it.
In sum, this is a handy and attractive watch that is quite informal in appearance and will require some patience to perform battery changes.
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Reviewed in the United States on April 8, 2026
★★★★★ 5
Simple, easy to read, dependable, and affordable: all the important stuff!
Color: Black/White/Black
Its just a fine time piece for daily wear. Its light, its super easy to read both day and night. Its comfortable and it looks good on your arm. What more can you ask of a watch? If you want "fancy" go buy some high dollar metal case thing and pay three or four (or more) times what this watch cost. If you want to know what time it is and not spend a bunch of money, this is your baby.
You can find cheaper watches, but you will not find a better watch at this price. I know, I have been wearing out watches for decades! My work is hard on watches. Things like using power tools, impact tools, hammers, welders, etc. These watches have never failed me and have lasted longer than watches costing far more.
I got my first Timex watch nearly sixty years ago. I am still buying them as I have found nothing else that is more accurate or holds up under harsh conditions any better.
You get what you pay for except with Timex. They give you MORE than you paid for.
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Reviewed in the United States on April 12, 2020
★★★★★ 5
I like the nylon strap.
Color: Black/White/Black
Watch itself is fine - doesn't stand out as something spectacular but is great for what it is. Definitely waterproof to about 30 feet deep - I've tested it snorkeling. The best thing is the strap - this nylon strap is much more comfortable than usual watch straps. There are a lot of nylon straps out there -- I think I prefer this one more than most of the others. the Velcro allows infinite adjustment, unlike straps with holes like a belt. And the way they did the looping of the band around itself seems to work really well. Better than another velcro strap I had many years ago ("the band") which was similar but didn't loop around itself the same way. This one is better - quicker and easier to put on and more easily adjustable.
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Reviewed in the United States on August 1, 2025
★★★★★ 5
Great purchase
Color: Black/White/Black, Color: Black/White/Black
The perfect watch for my eyes. White background with black hour and the second hand. Standard stuff…
It’s all about the simple things that matter to me.
The indigo green light might not appeal to everyone (look at the price). The band is fabric with Velcro to adjust. I’ve work this watch day and night. A near perfect fit. Easy to adjust and it’s comfortable for me, the look is just right for me. A no brainer to set up immediately.
Time (?) will tell about minute creep or over all durability,
A nice affordable watch that I I’ll wear everyday under all conditions.
If I loose it I’ll buy another one.
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Reviewed in the United States on February 10, 2026
★★★★★ 5
Inexpensive Box Checker That Works Great!
Color: Black/White/Black
Although I have a few watches I was looking for something that was more camping specific. Something that was EASILY legible to my 56 year old vision - both in daytime and night. I needed it to have some waterproofness ability as I would also using it for kayaking. I had a Timex Expedition watch with a black dial that I gave to my son as I wanted one with a white face which I find easier to read. I gave this watch some serious consideration after seeing so many YTbers give Timex field watches the thumbs up. I came upon this model and then when I saw one available under $30 it became a no-brainer! I used it recently for its intended purpose - a 3 day camping weekend. So here are my observations. The strap is probably the only negative to the watch for me as I have big hands and need to undo it completely to feed the strap through the buckle. A minor hindrance. Once its on it's light and comfortable. I may eventually change out the strap...or I may not. The watch is easily legible and nothing, I MEAN NOTHING, beats Timex's "Indiglo" feature for nighttime. Forget "watch lume" as nothing is a visibly legible at nights as "Indiglo". I have a Casio watch that has a light feature but that cannot hold a candle to this watch's nighttime ability. On a kayak trip, I overturned the kayak and the watch was in the water so it held up nicely for that. The watch is rated for 100M so that was nothing for it I know. It's hard to find a watch that does so much at this price point. I would definitely recommend it for someone looking for the features that it has in an inexpensive package.
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Reviewed in the United States on November 24, 2020