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Description
Mouse F8 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect specimens using EDTA or heparin as an anticoagulant and centrifuge at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed immediately or stored at -20°C or -80°C, but avoid repeated freeze-thaw cycles. Pre-Assay Preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let stand for 15 minutes to completely dissolve, then gently mix (concentration 40 ng/mL). Then dilute to the following concentrations: 40 ng/mL, 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 40ng/mL standard working solution into the first EP tube and mix thoroughly to make a 20ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Coagulation Factor VIII (F8) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Coagulation Factor VIII (F8) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Coagulation Factor VIII ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Factor VII (I FVIII) is an important blood clotting protein, also known as antihemophilic factor (AHF). Factor VIII is encoded by the F8 gene. Defects in this gene cause hemophilia A, a recessive X-linked clotting disorder. Factor VIII is produced in the sinusoidal cells of the liver and the endothelial cells outside the liver, and is found throughout the body. This protein circulates in the blood in an inactive form, bound to another molecule called von Willebrand factor, until an injury occurs that damages a blood vessel. In response to injury, factor VIII becomes activated and dissociates from von Willebrand factor. The active protein (sometimes written as factor VIIIa) interacts with another clotting factor called factor IX. This interaction triggers a cascade of additional chemical reactions that form a blood clot. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.62-40 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma |
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4.8 ★★★★★
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Product Reviews
★★★★★ 5
Matcha Powder
Flavor Name: Culinary, Size: 1.06 oz (Pack of 1)
Jade Leaf Matcha has become my go-to matcha powder, and I keep coming back to it time and time again. The quality is consistently great, with a fresh flavor and vibrant green color that mixes well into drinks and recipes. I use it regularly for matcha lattes, smoothies, and even baking, and it always delivers great results.
For a culinary-grade matcha, I think the flavor is excellent—not overly bitter and smooth enough to enjoy daily. It blends easily and provides the perfect matcha taste without breaking the budget. I’ve tried other brands over the years, but this is the one I continue to repurchase. If you’re looking for a reliable, high-quality matcha for everyday use, I highly recommend giving this one a try.
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Reviewed in the United States on June 7, 2026
★★★★★ 5
Good taste,
Flavor Name: Culinary, Size: 3.53 oz (Pack of 1)
This matcha has a nice, rich flavor and works really well for lattes. It mixes easily without clumps and the color is vibrant. Good quality for the price and perfect for daily use.
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Reviewed in the United States on April 26, 2026
★★★★★ 4
Matcha
Flavor Name: Culinary, Size: 1.06 oz (Pack of 1)
I haven’t decided if I really like Matcha, as I got this, just to find that out. I find that the packaging is interesting, and it is best, probably, to store it in the package it comes in. It has a mild flavor, and I am just making tea with it, as I don’t bake any more. It is a bright green powder with a smooth, mild taste, and in a clever package.
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Reviewed in the United States on June 7, 2026
★★★★★ 5
A Premium Matcha Experience for the True Connoisseur
Flavor Name: Culinary, Size: 1.06 oz (Pack of 1)
I recently had the pleasure of trying the Jade Leaf Matcha Organic Premium Ceremonial Grade Matcha Green Tea Powder, and it truly lives up to its reputation as a high-quality product. As someone who enjoys matcha regularly, I was eager to compare this offering to others on the market, and I have to say I was very impressed.
The first thing I noticed was the vibrant green color of the powder. It’s a rich, deep green that is indicative of the quality and freshness of the leaves. When prepared, the tea has a smooth, silky texture and a natural sweetness that makes it enjoyable both on its own and as an addition to smoothies or lattes. I appreciate that this matcha doesn’t have the bitterness that can sometimes accompany lesser-quality varieties, making it a perfect choice for ceremonial use or a relaxing daily treat.
As a ceremonial grade matcha, it’s clear that Jade Leaf has carefully sourced and processed the tea leaves. The flavor is balanced and complex, with grassy, vegetal notes followed by a subtle sweetness. It has the perfect level of umami, which is an essential characteristic of a high-quality matcha. I’ve tried a variety of brands, but this one stands out for its consistency and superior taste.
The packaging is also noteworthy. The resealable bag ensures the matcha stays fresh, which is crucial for preserving its delicate flavor and antioxidants. It’s easy to scoop out the right amount without making a mess, and the instructions on the back are clear and helpful for those new to preparing matcha.
Whether you’re a seasoned matcha drinker or new to the world of green tea, this Jade Leaf matcha provides a top-notch experience. I highly recommend it for anyone looking for an authentic, high-quality matcha that delivers excellent taste and a sense of calm and clarity with every sip.
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Reviewed in the United States on January 12, 2026
★★★★★ 5
The all day energy
Flavor Name: Culinary, Size: 1.06 oz (Pack of 1)
Great taste when you know how to make it..gives me full of energy, the smell.is fresh, the color is the perfect green, not grit and the price is reasonable. Drink it in a tea drink or smoothie . Smooth texture. Very drinkable.
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Reviewed in the United States on May 26, 2026