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Description
Human CRN ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a corin (CRN) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of corin (CRN) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Corin ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Corin protein (CRN) belongs to the type II transmembrane serine protease family with a molecular weight of approximately 170-200 kDa. Its N-terminus is located inside the cell and has a transmembrane region. The extracellular region contains two curled structures (Frizzled, Fz), 8 repeats of low-density lipoprotein receptor structures (LDLR), a scavenger-like receptor structure (SR) and a conserved C-terminal protease (Protease) region. The protease domain has a conserved structure unique to trypsin-like proteases, including enzyme active groups containing histidine (H), aspartic acid (D) and serine (S). | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates and other biological fluids |
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4.0 ★★★★★
Based on 30 reviews
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Product Reviews
★★★★★ 5
Excellent espresso tool
Color: Black
Very nicely made and works as intended. Also comes with extra needles. Highly recommend.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 17, 2026
★★★★★ 5
WDT Tool: The Needle in My Espresso Haystack
Color: Black, Color: Black
I kind of went down a deep dive researching these WDT (Weiss Distribution Technique) tools, and I have to say, this one hits the sweet spot! The needle size is just right, and the shape is spot on for what I need. Plus, it’s a nice perk that you can remove and replace the needles. I’ve tried using needles and bobby pins in the past, but they were too thick and just ended up creating their own channels—talk about a brewing disaster!
Now, here’s the twist: even though it comes with seven needles installed (and more replacements), I find it a bit overkill. With all those needles, it just traps the grinds in the center, pushing stuff around like it’s playing a game of “don’t let the coffee escape.” So, I stick with just three needles in a triangle formation for optimal distribution. Pro tip: don’t forget to adjust the depth as you use it to ensure you’re getting the best coverage of your coffee grounds!
The build quality is actually pretty impressive. It feels like well-constructed metal components that could survive a small espresso war. Plus, it's more affordable than some of those fancy options out there—because who needs a second mortgage for great coffee?
It looks quite nice on my counter, and the little stand is a handy extra touch.
Overall, if you’re looking to up your espresso game and avoid the chaos of uneven distribution, this WDT tool is worth considering. Just remember: less is sometimes more when it comes to needles!
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Reviewed in the United States on October 20, 2024
★★★★★ 5
Love this thing!!!
Color: Black, Color: Black
Hey, coffee lovers! ☕️ I recently got my hands on the WDT Espresso Distribution Tool Espresso Stirrer Tools for Barista, and let me tell you, it's a game-changer! 🙌 I'm absolutely thrilled with this amazing tool and can't recommend it enough to fellow coffee enthusiasts and professional baristas alike.
First things first, the build quality of this espresso stirrer is top-notch! 💪 The aluminum alloy handle not only adds durability but also gives it a sleek and professional look, especially in the matte black finish. Holding this tool feels great, and the ergonomic design ensures a comfortable grip even during those long brewing sessions.
The 0.35mm 7 needles are perfectly spaced, allowing for precise and consistent distribution of coffee grounds in the portafilter. This means a more even extraction and an enhanced flavor profile in every sip. And guess what? You get 7 extra replaceable needles as a bonus! 🎉 This ensures the longevity of the tool and easy replacement when needed.
But wait, there's more! The included stand is a fantastic addition to this package. 🎁 It not only serves as a convenient storage solution but also adds a touch of elegance to my coffee setup. No more rolling around and cluttered workspace. Everything stays in place, thanks to the stand!
Using the WDT Espresso Distribution Tool is a breeze! ⚡️ The precisely engineered needles and ergonomic design make it effortless to distribute the coffee grounds evenly. Whether you're a pro barista or a home brewing enthusiast, this tool will take your espresso game to new heights. Trust me on this one!
In conclusion, the WDT Espresso Distribution Tool Espresso Stirrer is an absolute must-have for any coffee lover out there. 💯 Its top-notch build quality, precision, and thoughtful design will bring joy to your coffee routine. So, don't wait any longer! Treat yourself and elevate your espresso-making experience with this amazing tool. Cheers! 🥳☕️
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Reviewed in the United States on June 12, 2023
★★★★★ 5
Easy to use and affordable - great value for money!
Color: Black
I recently purchased the Nessus WDT Espresso distribution tool and I am thoroughly impressed with its performance. Assembling it was a breeze, and it came with extra needles which is always a plus. The tool is easy to clean, which is important to me because I like to keep my equipment in top condition. The price was very fair compared to other similar products on the market, making it an even better value for money.
One thing that really stood out to me was the brush that collects the coffee grounds. It's incredibly easy to clean, which makes maintenance a breeze. I've had my share of espresso machines in the past, and I can tell you that this one is a keeper. I would definitely recommend it to anyone looking for a high-quality distribution tool at an affordable price
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 16, 2025
★★★★★ 5
This WDT is perfect!
Color: Black
I love this WDT! I had to look at the photos of the product to figure out how to "install" the needles. It took just a little patience to align the needles with the holes. I think the design is flawless. You can choose how many needles to use and the needle length is perfect. The needles are shorter than my previous WDT, and I have more control with the shorter needles. It's easy to slip the tool into its holder and the brush keeps the needles clean. I'm very particular, and I think this tool is perfect! I went to the Nessus page to see what other espresso tools they have because I like this tool so much, and the price is great! Unfortunately, I didn't see other tools.
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Reviewed in the United States on February 8, 2026
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