SKU: 43306322308

Cathepsin B His tag Protein, Human

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Description

Cathepsin B His tag Protein, HumanProduct Specification Species Human Synonyms APP secretase (APPS), Cathepsin B1, CPSB Accession P07858 Amino Acid Sequence Met1 Ile339, with C 10*His Expression System HEK293 Molecular Weight Predicted MW: 39. 5 kDa Observed MW: 43 kDa Purity >95% by SDS PAGE Endotoxin <1EU g Conjugation Unconjugated Tag His Tag Physical Appearance Lyophilized powder Storage Buffer 0. 2M PBS, pH7. 4. Reconstitution Reconstitute no more than 1 mg mL according to the

Product Specification


Species Human
Synonyms APP secretase (APPS), Cathepsin B1, CPSB
Accession P07858
Amino Acid Sequence

Met1-Ile339, with C-10*His

Expression System HEK293
Molecular Weight

Predicted MW: 39.5 kDa Observed MW: 43 kDa

Purity >95% by SDS-PAGE
Endotoxin <1EU/μg
Conjugation Unconjugated
Tag His Tag
Physical Appearance Lyophilized powder
Storage Buffer

0.2M PBS, pH7.4.

Reconstitution

Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation.

Stability & Storage


12 months from date of receipt, -20 to -70 °C as supplied.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
1 week, 2 to 8 °C under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.

Background

Cathepsin B belongs to a family of lysosomal cysteine proteases known as the cysteine cathepsins and plays an important role in intracellular proteolysis. In humans, cathepsin B is encoded by the CTSB gene. Cathepsin B is upregulated in certain cancers, in pre-malignant lesions, and in various other pathological conditions. Cathepsin B may enhance the activity of other proteases, including matrix metalloproteinase, urokinase (serine protease urokinase plasminogen activator), and cathepsin D, and thus it has an essential position for the proteolysis of extracellular matrix components, intercellular communication disruption, and reduced protease inhibitor expression. Cells may become carcinogenic when cathepsin B is unregulated. Cathepsin B has been proposed as a potentially effective biomarker for a variety of cancers. Overexpression of cathepsin B is correlated with invasive and metastatic cancers.

Protocol

Assay protocol
Principle: Measured by its ability to cleave the fluorogenic peptide substrate, Z-LR-AMC.
Materials
1.Protein Activation Buffer: 25 mM MES, 5 mM DTT, pH 5.0
2.Assay Buffer: 25 mM MES,0.02% Brij-35, pH 5.0
3.Human Cathepsin B, His tag)
4.Substrate: Z-LR-AMC (ES008) 
5.96 ELISA Removable Plate, Black, High binding (GENEVER, Catalog # GMO2-96H)
6.Plate Reader (PerkinElmer, excitation 380 nm and emission 460 nm)
Produce
1.Dilute Cathepsin B to 10 μg/mL in Activation Buffer.
2.Incubate at room temperature for 15 minutes.
3.Dilute Cathepsin B to 0.4, 0.2 and 0.1 μg/mL in Assay Buffer.
4.Dilute substrate to 80 μM in Assay Buffer.
5.Load 50 μL of the 0.4, 0.2 and 0.1 μg/mL Cathepsin B in a black well plate, and start the reaction by adding 50 μL of 80 μM Substrate. Include a Substrate Blank containing 50 μL Assay Buffer and 50 μL of 80 μM Substrate.
6.Read at excitation and emission wavelengths of 380 nm and 460 nm (top read), respectively, in kinetic mode for 5 minutes. Calculate specific activity:

Specific Activity (pmol/min/µg) =

Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)

amount of enzyme (µg)

*Adjusted for Substrate Blank
**Derived using calibration standard 7-amino-4-Methyl Coumarin

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SKU: 43306322308

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★★★★★ 4
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Reviewed in the United States on June 7, 2026
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Karen Pezdek
Cuba, US
★★★★★ 5
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Size: Basic Frother, Color: Black
I love this mixer. I don't know how we got along before we had this. We have had this small hand mixer for 2 years, using it several times every day, day after day and it finally stopped working and I am ordering another one right now. I can't live without it. I make our bullet coffee with it. My wife makes her morning shake with it. I could go on and on but I need to get another one ordered right now.
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Reviewed in the United States on May 26, 2026
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A. Yang
Alexandria, US
★★★★★ 5
Works perfectly!
Size: Basic Frother, Color: Silver
The first unit I got, the charging LED was not working so I don't know if the unit is charged. I returned the unit back to Amazon and got a brand new unit the next day. Great job, Amazon! The 2nd unit has been perform perfectly. I use it to whisk eggs in the morning to make French toasts, waffles, pancakes. All comes out perfect! Also, the mike froth for my morning latte and cappuccino. Don't under estimate this small unit, the spin is fast for high!!! I saw some negative review on the durability of the unit. I hope this is not the case. I'll update if anything changes. As for now, highly recommended!!!
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Reviewed in the United States on May 18, 2026
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★★★★★ 5
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This is frother is compact what does that meanand strong, and easy to keep
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