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Description
Cytochrome C Recombinant Rabbit mAb (S-2422-56)Product Specification Host Rabbit Antigen Cytochrome C Synonyms Cytochrome c; CYC; CYCS Immunogen Synthetic Peptide Location Mitochondrion Accession P99999 Clone Number S 2422 56 Antibody Type Recombinant mAb Isotype IgG Application WB, IHC P Reactivity Hu, Ms, Rt, Mk Positive Sample HeLa, HepG2, Molt 4, SH SY5Y, C2C12, mouse brain, mouse kidney, C6, rat brain, rat kidney, COS 7 Predicted Reactivity Bv, Cz, Dg, Hr, RhMk Purification Protein A
Product Specification
| Host | Rabbit |
| Antigen | Cytochrome C |
| Synonyms | Cytochrome c; CYC; CYCS |
| Immunogen | Synthetic Peptide |
| Location | Mitochondrion |
| Accession | P99999 |
| Clone Number | S-2422-56 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HeLa, HepG2, Molt-4, SH-SY5Y, C2C12, mouse brain, mouse kidney, C6, rat brain, rat kidney, COS-7 |
| Predicted Reactivity | Bv, Cz, Dg, Hr, RhMk |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:10000 | Hu, Ms, Rt, Mk |
| IHC-P | 1:2000 | Hu, Ms, Rt |
Background
Cytochrome c is a small, highly conserved heme-containing electron-transfer protein located in the intermembrane space of mitochondria, where it shuttles electrons between Complex III and Complex IV of the respiratory chain via reversible Fe²⁺/Fe³⁺ redox changes in its covalently attached heme group, and, beyond its bioenergetic role, its release into the cytosol in response to apoptotic stimuli nucleates the apoptosome by binding Apaf-1, making it a central switch between life-sustaining respiration and programmed cell death.
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