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Description
Rat TFR2 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be ultrasonically disrupted or repeatedly frozen and thawed. Finally, the homogenate is centrifuged at 5000×g for 5-10 minutes and the supernatant is collected for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a transferrin receptor 2 (TFR2) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of transferrin receptor 2 (TFR2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Transferrin Receptor 2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Transferrin receptor 2 (TFR2) is a protein encoded by the TFR2 gene. This protein is involved in the uptake of transferrin-bound iron into cells via endocytosis, although its role is less pronounced than that of transferrin receptor 1. This gene, a member of the transferrin receptor family, encodes a single-pass, type II membrane protein with a protease-associated (PA) domain, an M28 peptidase domain, and a transferrin receptor-like dimerization domain. This protein mediates the cellular uptake of transferrin-bound iron, and mutations in this gene are associated with hereditary hemochromatosis type III. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates and other biological fluids |
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4.4 ★★★★★
Based on 5 reviews
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Product Reviews
★★★★★ 3
Liner seems good, but releases strong fumes
Color: Taupe, Size: 12-Inch x 20 Feet
The liner is quite functional, it looks fine and is manufactured in a straight/square style (the other thicker foam style liner is not straight and is hard to cut square).
The downside, with the 2 rolls that I got, is that the liner has a nasty off-gassing of fumes, so I suggest cutting it to size, and then putting it through a wash cycle (dishwasher or clothes washer) and then putting it in place after drying. If you don't do this, then those of you who are sensitive to these kinds of fumes, might have to unload the items and wash the liner at a later time.
Many are oblivious and won't notice the fumes (at first or ever) and they just go on, never making the connection as to why their kitchen stinks, or they have headaches (or imagined allergies, etc.). It took me days to figure it out. If you have a new roll of the liner, just smell it, and realize that if you don't wash this material, those fumes will be leaching out into your cupboard for a while (perhaps longer). For those that can't smell it, then I guess you'll be quite happy about that.
While the liner looks fine, and easily cuts square, I can't give this a higher rating as the washing effort adds so much effort to the process.
More and more items produce these kinds of fumes and I think reviewers should mention this as part of their overall rating.
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Reviewed in the United States on July 13, 2014
★★★★★ 5
A PLACE FOR YOUR HEADSET
Size: Small Storage Area, Size: Small Storage Area
This holder is fantastic, if you have the steel series headset and extra batteries it's perfect you'll love it, but would like to see a white version as well.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 23, 2026
★★★★★ 2
Not quite right
Size: Small Storage Area
this is "as advertised" but
a simple extra ridge of plastic would have made it perfect.
the issue people are having with the arctis pro, the thing that makes you want a stand for it, is the base station is a little too light, you can push it when you go to use the knob's integrated button.
Issue here is the fit of the base is loose enough, you can still push the base out of the back. So it looks nice, and gives you a place to put the headphones and some other desk clutter, but it doesn't REALLY solve the problem.
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Reviewed in the United States on March 13, 2026
★★★★★ 3
3D Printed (Not Mentioned) and Base is Way Too Loose
Size: Small Storage Area, Size: Small Storage Area
I bought this to clean up my desk setup for my SteelSeries Nova Pro Wireless, and while it looks decent from a distance, there are two major issues that weren't clear from the product page.
First, this is a 3D-printed product. Nowhere in the Amazon description or photos does it state that this is 3D printed. You can clearly see the layer lines and "stair-stepping" on the curves. While I don't mind 3D printing in general, for this price point, I expected a standard injection-molded plastic finish. The product page feels a bit misleading by omitting this detail.
Second, the fit is very poor. The "integrated dock" for the GameDAC station doesn't actually lock into place. There is no snap or friction fit; it sits so loosely that you can slide the DAC out of the front or back with the single push of a finger. This makes it feel unstable, especially when you are trying to use the volume dial or swap out batteries.
Bottom Line: It’s a nice idea and fits the aesthetic of the headset, but the execution feels like a prototype rather than a finished consumer product. If you're looking for a secure "lock-in" feel for your expensive base station, this isn't it.
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Reviewed in the United States on January 12, 2026
★★★★★ 5
High quality stand
Style: Swivel Base, Color: Silver, Style: Swivel Base, Color: Silver
The mStand 360 is an amazing stand. I love how it is built from a single piece of aluminum and looks amazing on my desk. It fits my Macbook Pro 15" extremely well and raises it up to the height of my monitor easily. Not only does it help me improve my posture, but it also neatens up my desk significantly. I can easily fit an external keyboard under my Macbook when I am not using it, clearing up space. The hole in the back is also useful for bunching cables together that go directly into my Macbook, keeping my cables off the floor when I take my Macbook to go somewhere.
The stand feels very stable, with no chance of my Macbook falling off. The aluminum C shape does have a slight bend on it if you put extra weight on it, but all in all, it feels very sturdy. The rubber padding on the stand also keeps the stand from scraping or damaging any part of my laptop and I feel very comfortable trusting the stand with my laptop. I would not recommend typing on a laptop keyboard while it is on the stand, but it works great with external keyboards.
The swivel feature met my expectations, allowing the laptop to be easily turned. Thankfully, it was not too easy to turn and my laptop does not turn itself when in the stand. It is a nice feature to have, especially because I paid only 5$ extra for it (the price for the swivel stand seems to have increased since then).
I am very happy with my purchase, even though it did seem a little expensive. I am wholly satisfied that I spent the extra 20$ to get a great laptop stand that will probably last for years.
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Reviewed in the United States on August 18, 2016