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Description
Rat IFN-γ OneStep ELISA KitProduct Specification Antigen IFN Immunogen Recombinant Protein Antibody Type Recombinant mAb Reactivity Rt Purification Protein A Stability & Storage 2 to 8 C as supplied. Kit Precision Intra assay: 3. 1% Inter assay: 3. 9% Sample type Cell culture supernatant, Serum, Plasma Assay type Sandwich (quantitative) Sensitivity 0. 846 pg ml Range 2. 34 pg mL 150 pg mL Recovery Cell culture supernatant: 92% Serum: 81% Plasma: 101% Assay time 60 minutes
Product Specification
| Antigen | IFN-γ |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Rt |
| Purification | Protein A |
| Stability & Storage | 2 to 8 °C as supplied. |
Kit
| Precision | Intra-assay: 3.1%； Inter-assay: 3.9% |
| Sample type | Cell culture supernatant, Serum, Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 0.846 pg/ml |
| Range | 2.34 pg/mL – 150 pg/mL |
| Recovery | Cell culture supernatant: 92% Serum: 81% Plasma: 101% |
| Assay time | 60 minutes |
| Species reactivity | Rat |
Background
Interferon gamma (IFNG or IFN-γ) is a dimerized soluble cytokine that is the only member of the type II class of interferons. Through cell signaling, interferon gamma plays a role in regulating the immune response of its target cell. A key signaling pathway that is activated by type II IFN is the JAK-STAT signaling pathway. IFNG plays an important role in both innate and adaptive immunity. Type II IFN is primarily secreted by adaptive immune cells, more specifically CD4+ T helper 1 (Th1) cells, natural killer (NK) cells, and CD8+ cytotoxic T cells. The expression of type II IFN is upregulated and downregulated by cytokines. By activating signaling pathways in cells such as macrophages, B cells, and CD8+ cytotoxic T cells, it is able to promote inflammation, antiviral or antibacterial activity, and cell proliferation and differentiation. Type II IFN has played a role in the development of cancer immunotherapy treatments due to its ability to prevent tumor growth.
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