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Description
Mouse TL ELISA KitProduct Specification Sensitivity 9. 375ng ml Species Reactivity Mouse Theory This kit uses competitive ELISA assay, and the experimental duration is 2 hours. The plates supplied in the kit have been pre coated with TC. The TC to be tested in the sample or standard will compete with the TC previously coated on the microplate for binding to a fixed amount of biotin labeled anti TC antibody. Free components are washed away. HRP streptavidin (SABC) is
Product Specification
| Sensitivity | 9.375ng/ml | ||||||||||||||||||||||||||||||||||
| Species Reactivity | Mouse | ||||||||||||||||||||||||||||||||||
| Theory | This kit uses competitive ELISA assay, and the experimental duration is 2 hours. The plates supplied in the kit have been pre-coated with TC. The TC to be tested in the sample or standard will compete with the TC previously coated on the microplate for binding to a fixed amount of biotin-labeled anti-TC antibody. Free components are washed away. HRP-streptavidin (SABC) is added, and biotin specifically binds to streptavidin to form an immune complex. The unbound conjugate was washed off and TMB chromogenic substrate was added. The TMB became blue under the catalysis of horseradish peroxidase (HRP) and turned yellow after the reaction stop solution was added. The OD value was measured at 450nm with a microplate reader. Using curve equation software, the OD450 value of the sample was compared to the standard curve to determine the concentration of TC in the sample. There is an inverse proportion between the concentration of the target substance and the OD450 value. | ||||||||||||||||||||||||||||||||||
| Synonym | Mouse TC (Total cholesterol) ELISA Kit | ||||||||||||||||||||||||||||||||||
| Detection Type | Competition Law | ||||||||||||||||||||||||||||||||||
| Composition |
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| General Notes | 1. When using different kits, it is necessary to mark them first to prevent the components from being mixed, resulting in experimental failure. 2. After the kit is opened, please refer to the component storage conditions table for the storage conditions of the enzyme plate and standard product (the activity will decrease after moisture). 3. Please use a sterile disposable tip to suck the reagent. After use, the reagent bottle cap must be tightened to prevent microbial contamination and evaporation. 4. When washing the plate manually, add the tip or dropper of the washing liquid and do not touch the hole of the enzyme labeled plate. Inadequate washing or contamination tend to cause false positives and high backgrounds. 5. During the testing process, please prepare the reagents required for the next experiment in advance, and add the reagents to the plate holes in time after washing the plate to prevent the plate holes from drying out and causing testing failure. 6. Do not use reagents from other batches of kits or reagents from other sources for this kit without confirmation. 7. Do not reuse disposable tips to avoid cross-contamination. 8. After the sample addition is completed, the film is applied to prevent the sample from evaporating during the incubation process, and the incubation process is completed at the recommended temperature. 9. Please wear laboratory coats, masks, gloves, etc. during the test, and do a good job of protection. Especially when testing blood or other body fluid samples, please follow the national biological laboratory safety protection regulations. |
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| Instructions | Serum, plasma, cell culture supernatant, cell lysate and other fluid samples | ||||||||||||||||||||||||||||||||||
| Storage Temp. | Unopened kit, stored at 2-8 °C, shelf life for one year. | ||||||||||||||||||||||||||||||||||
| Test Range | 15.625-1000ng/ml |
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4.9 ★★★★★
Based on 11 reviews
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Product Reviews
★★★★★ 5
Honestly the best
Size: 8 Ounce (Pack of 1)
I have pretty sensitive skin and have keratosis pilaris on my arms. This is honestly the best lotion I’ve ever used and I’ve tried many many lotions. My skin is soft but not greasy. My arms feel soft which is a huge deal. I’ve been using it for months now and I don’t think I’ll ever use anything else.
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Reviewed in the United States on April 29, 2026
★★★★★ 5
Extremely Helpful for Dry, Irritated Skin but Personally Too Heavy for My Face
Size: 1.35 Fl Oz (Pack of 1), Style: Cicaplast Baume B5 Balm
This is one of those products that I completely understand the hype around because it truly does work very well for dry, irritated, over-processed, or compromised skin areas. The texture is rich, soothing, and very protective without having a strong fragrance, which I appreciate because heavily scented skincare products usually irritate my skin immediately.
I have found this especially helpful for dry patches, irritation from weather, over-exfoliation, and areas where the skin barrier simply feels stressed or uncomfortable. A very small amount goes a long way and the tube itself lasted much longer than I expected.
That being said, for me personally this product is simply too heavy to use regularly on my face because I am somewhat breakout-prone with richer creams. While it feels incredibly soothing, I personally prefer using it more selectively on very dry or irritated areas rather than as an all-over facial moisturizer.
The packaging arrived perfectly sealed with no leaking or damage during shipping and the product looked exactly as expected upon arrival. For the amount you receive and how little is needed per use, I also think the value is very good compared to many other barrier repair creams.
Overall, I absolutely understand why so many people love this product and I do think it is excellent for repairing and protecting irritated skin — I just personally have to be careful about where and how heavily I apply it.
#FoundItOnAmazon #AmazonFinds #Skincare #SkinBarrier #LaRochePosay #SensitiveSkin #DrySkin #BeautyFavorites #EverydaySkincare #FrenchPharmacy #SelfCare #LuxeLife
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Reviewed in the United States on May 11, 2026
★★★★★ 5
Excellent skincare product
Size: 1.35 Fl Oz (Pack of 1), Style: Cicaplast Baume B5 Balm
I am very happy with this product. I have been using it on irritated and dry skin, and I noticed a big improvement in a short time. It has a smooth texture, absorbs well, and does not feel greasy. It helps calm the skin quickly and restores the skin barrier. I have used it both on my face and on small sensitive areas of the body, and it works very well in both cases. It is definitely a skincare essential that I will always keep in my routine. I highly recommend it.
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Reviewed in the United States on May 30, 2026
★★★★★ 5
Worth the hype
Size: 1.35 Fl Oz (Pack of 1), Style: Cicaplast Baume B5 Balm
I’ve seen La Roche-Posay Cicaplast Balm B5 recommended countless times online, so I finally decided to try it for irritated skin and a damaged skin barrier. It absolutely lives up to the hype for me. The texture is very rich and moisturizing, but it feels extremely soothing on dry, overtreated, or sensitive areas. I’ve used it on my face, hands, and other irritated patches of skin, and it really helps calm everything down.
The quality feels excellent, which is what I expect from La Roche-Posay. A little goes a long way, so I think it’s a very good value for the price. I also like that it’s versatile enough for the whole family. I’ve even shared it with my daughter, and we both love having it around for dry or irritated skin. Definitely one of those products I can see myself repurchasing regularly.
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Reviewed in the United States on May 8, 2026
★★★★★ 5
Amazing Skin Recovery Balm – Hydrating, Soothing, and Instant Tone-Up Effect!
Size: 1.35 Fl Oz (Pack of 1), Style: Cicaplast Baume B5 Balm
I bought this after an AI recommendation while looking for something to help my skin recover after professional dermatology treatments, and I’m so glad I did. This balm is incredibly moisturizing and soothing without feeling heavy or greasy. My skin barrier felt calmer almost immediately, and it really helped speed up the recovery process.
One thing I love is the subtle tone-up effect — it makes my skin look brighter and more even right after application, almost like a natural glow without makeup. It’s perfect for post-treatment care, irritation, dryness, and overall skin repair.
A little goes a long way, and it has become a staple in my skincare routine. Highly recommend this to anyone needing skin recovery support and a healthy-looking glow. I will definitely repurchase!
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Reviewed in the United States on April 30, 2026
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