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Description
Human CLEC4G ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000 × g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash the collected cells three times with ice-cold PBS and resuspend them in 150-200 μL of PBS per 1 × 10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500 × g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a c-type lectin domain family 4 member G (CLEC4G) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of c-type lectin domain family 4 member G (CLEC4G) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human c-type lectin domain family 4 member G ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | C-type lectin domain family 4 member G (CLEC4G), also known as LSECtin (liver and lymph node sinusoidal endothelial cell C-type lectin), is a member of the second subgroup of the C-type (Ca2+-dependent) lectin superfamily. It is an approximately 40 kDa, single-pass, type II transmembrane glycoprotein with a length of 293 amino acids (aa). It contains a short N-terminal cytoplasmic tail (aa 1-31) and a 21-aa transmembrane region. Its extracellular region includes two N-linked glycosylation sites (a73 and 159), a coiled-coil neck domain (a96-136), a C-type lectin-like domain (CTLD) found in DC-SIGN and DC-SIGN receptors (a165-289), and a C-terminal Ca2+-dependent carbohydrate recognition domain (C-type CRD). | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates, cell lysates and other biological fluids |
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4.7 ★★★★★
Based on 14 reviews
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Product Reviews
★★★★★ 4
Element of its stitched pattern may matter to some.
Color: Light Grey, Size: Queen (90" x 90"), Color: Light Grey, Size: Queen (90" x 90")
The blanket color was as expected, its fabric I believe will keep you warm, and no material odd scents which is good. Not a flaw, but what might matter to some, at least for me, is that in its stitched pattern it has a repeated section that has loose threading. I noted close up of that in my photos. I can see that element of the blanket being something that may snag on things sometimes over time from items you might set on this bed blanket. Nothing to cut much of a rating on but I wouldn’t of wanted this blanket due to that if I saw that design feature up close beforehand.
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Reviewed in the United States on June 1, 2026
★★★★★ 5
🛌 Ultimate Lightweight Summer Comfort!
Color: Pure White, Size: King (104" x 90")
🧶 Beautiful and Soft Waffle TextureThe 100% cotton construction offers a wonderfully soft, textured feel that adds a touch of classic elegance to the bedroom. It feels exceptionally gentle against the skin and provides the perfect amount of cozy texture without any scratchiness.
🍃 Exceptional Breathability and WeightThe lightweight waffle weave allows for maximum airflow, making it absolutely perfect for hot sleepers and warm summer nights. It keeps the body comfortably cool while still providing that comforting weight of a real bed blanket.
📐 Generous and True King Size FitDraping beautifully over a large king-size bed, the dimensions provide excellent coverage on all sides without pulling or shifting. It acts as an ideal standalone layer for the summer or a sleek layering piece under a comforter during cooler months.
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Reviewed in the United States on June 2, 2026
★★★★★ 5
Super soft blanket
Color: Polar Blue, Size: Full/Queen (90 in x 90 in), Color: Polar Blue, Size: Full/Queen (90 in x 90 in)
It turned out nicer than I expected, what a surprise. Very light and soft, slightly stretchy. It felt high quality and the color complimented the room perfectly. My mom even wanted to take it to the paint store and find out what color it is so she can paint her room the same color. Great blanket.
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Reviewed in the United States on June 1, 2026
★★★★★ 5
Nice blanket
Color: Southern Blues, Size: Full/Queen (90 in x 90 in), Color: Southern Blues, Size: Full/Queen (90 in x 90 in)
This is a really nice blanket. If fits on my bed very well. It went through the laundry really nice and didn’t fade or ripper. Have any strings come undone. I like the very light green color. My pictures aren’t great, but one side is the underneath side, the white and then the light green is the other picture. This is well-made you can see in the picture up close that it’s so really well. All layers are sewn together, so you do not have bunching up when you wash the blanket. It is super soft with a good weight. It’s not super lightweight, but it’s not heavy at all. Overall, I’m very pleased with it and I would recommend.
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Reviewed in the United States on May 29, 2026
★★★★★ 5
Soft, Lightweight, and Perfect for Year-Round Comfort
Color: Polar Blue, Size: King (90 in x 108 in)
This Berkshire 3-layer fleece blanket is exactly what I was hoping for. It is incredibly soft and has a cozy cotton-like feel without being too heavy or bulky. The king size is generous and fits the bed nicely, but it is also comfortable enough to use on the couch.
I really like that it feels warm and breathable at the same time, so it works well for different seasons. The Polar Blue color is soft and pretty, and the blanket has a nice textured look that makes it feel a little more elevated than a basic fleece blanket.
It also washes well, which is a huge plus. After washing, it still feels soft and comfortable, and it did not lose its shape. Overall, this is a great blanket if you want something cozy, lightweight, and easy to care for. I would definitely recommend it.
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Reviewed in the United States on May 10, 2026
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