SKU: 32645012309

UA-Glo Luminescent Mycoplasma Detection Kit

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Description

UA-Glo Luminescent Mycoplasma Detection KitProduct Specification Synonyms Mycoplasma luciferase detection kits Stability & Storage Dry ice transport. 20 storage is protected from light, and the validity period is 12 months Background The UA Glo Mycoplasma Detection Kit is used for the qualitative detection of mycoplasma contamination in cell cultures. Over 95% of mycoplasma contamination in cell cultures is caused by Mycoplasma fermentans, M. orale, M. pirum, M. hyorhinis, M. hominis, M.

Product Specification


Synonyms Mycoplasma luciferase detection kits
Stability & Storage

Dry ice transport. -20℃ storage is protected from light, and the validity period is 12 months

Background

The UA-Glo Mycoplasma Detection Kit is used for the qualitative detection of mycoplasma contamination in cell cultures. Over 95% of mycoplasma contamination in cell cultures is caused by Mycoplasma fermentans, M. orale, M. pirum, M. hyorhinis, M. hominis, M. salivarius, M. arginine, and Acholeplasma laidlawii. Live mycoplasmas produce kinases that are not expressed by eukaryotic cells, which can convert ADP into ATP. The Mycoplasma Detection Kit provides ADP and other substrates required for mycoplasma kinases to convert ADP into ATP, and then detects the generated ATP via a luciferase luminescence method to confirm whether there is mycoplasma contamination in the cell culture. This kit can quickly detect the eight aforementioned species as well as many other common live mycoplasma strains.

Components

Size

component

10T

Mycoplasma luciferase detection reagent 0.5ml

Mycoplasma luciferase detection reagent 0.1mL

25T

Mycoplasma luciferase detection reagent 1.25ml

Mycoplasma luciferase detection reagent 0.25mL

50T

Mycoplasma luciferase detection reagent 2.5ml

Mycoplasma luciferase detection reagent 0.5mL


Protocol

1. Sample Preparation for Cell Culture Medium to be Tested

  1. Take 1 mL of cell culture medium from cells in the logarithmic growth phase.

  2. Centrifuge the cell culture medium at room temperature (200 g) for 5 minutes. Transfer approximately 800 μL of the supernatant to a new centrifuge tube, making sure not to include any cell debris at the bottom.

  3. Use the prepared cell culture medium for the mycoplasma detection with luciferase assay.

2. Luciferase Mycoplasma Detection

  1. Add 50 μL of the cell culture medium sample to a white opaque 96-well plate.

  2. Equilibrate the luciferase mycoplasma detection reagent and substrate to room temperature (22°C-25°C), and mix gently.

  3. Add 50 μL of the luciferase mycoplasma detection reagent to the 50 μL of the cell culture medium sample.

  4. Mix by gentle shaking for 10 seconds, incubate at room temperature for 5 minutes, and then read the fluorescence value (Reading 1).

  5. Add 10 μL of the luciferase mycoplasma detection substrate.

  6. Mix by gentle shaking for 10 seconds, incubate at room temperature for 10 minutes, and then read the fluorescence value (Reading 2).

3. Results
The ratio of Reading 2 to Reading 1 is used to determine if mycoplasma contamination is present.

 

The ratio of reading 2 to reading 1

Results Explanation and Recommendations

< 1

No Mycoplasma contamination

1-1.2

Possible Mycoplasma contamination. The cells were isolated and tested again after culture for 24-48hr.

> 1.2

Mycoplasma contamination



Guidelines

  • Aliquot Storage: Aliquot and store the reagents according to the instructions at -20°C to ensure reagent stability.

  • Freeze-Thaw Stability: Reagents stored at -20°C, protected from light, can be freeze-thawed twice without affecting the test results.

  • Sample Collection: Collect samples from cells in the logarithmic growth phase for testing. Newly passaged cells should be tested 24-48 hours after subculture.

  • Luciferase Reaction Sensitivity: The luciferase reaction is sensitive to temperature changes. Reagents and samples should be equilibrated to room temperature (22°C-25°C), and the temperature should remain constant (±1°C) throughout the test.

  • For Research Use Only: This product is intended for research purposes only.

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SKU: 32645012309

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Verified Purchase
Melissa Williams
Lexington, US
★★★★★ 4
4.25 stars
Format: Kindle
Vale is an 8 month pregnant omega working as a waitress at a strip club and a cam girl. She starts to get very creepy vibes from a regular at the club, and her baby daddy ghosted her. She has had an online relationship with a man named Bishop through her cam girl status. One night, bishop was paying to watch her sleep and ansthe creepy regular Andrew break in and watch her sleep he tells vale to come to him at his business now. She flees and finds herself at a large security company with some.hot of alphas who are there to help her. This imegaverse is a little different than I have read, but I am thoroughly enjoying it. Vale is not a traditional omega she was raised by a single beta mom, and the alphas are not normal alphas they have never really loved pack life. But they are ruthless mercenaries. They need her, and she needs them. I love the aspect of the stalker and now the plot twists at the end, so so good. Sometimes, it seemed a little slow and stale mated, but since this a duet, I think It was just her starting to have Vale get to know her alpha suitors. Cliffhanger for sure with this one.
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Reviewed in the United States on September 9, 2024
A
Verified Purchase
Austin & Cambria
Charlottesville, US
★★★★★ 5
That ending 😫
Format: Kindle
I fell into a false sense of security and really thought this was gearing towards a happy ending. Then I realized there’s no work they don’t punish Andrew. I really liked Vale’s character. I don’t normally read books with pregnancy but going into this knowing she was pregnant made it more enjoyable for me. I loved Bishops devotion to her and her happiness. I also loved that Holt and Mercy couldn’t fight their attraction to her. I love scent matches so very much. I’m so curious to see how this duet will end up. And I need to pay more attention and notice that a book I’m starting is a duet to begin with lol
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Reviewed in the United States on February 21, 2025
S
Verified Purchase
Sarah A
Massapequa, US
★★★★★ 5
oh wow
Format: Kindle
I just knew there was something about Cooper! I’m wondering if he’s about to be included but damn I’m glad he’s at least not a rapist and creepy guy, he just got called on assignment and had to go! This should be interesting! She’s gonna run and then what’s his face is gonna grab her. I’m worried! Wow that was a great book and cliffhanger! Loving this!
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Reviewed in the United States on December 27, 2025
A
Verified Purchase
Ashley Morgan
Phoenix, US
★★★★★ 5
ABSOLUTELY A MUST for Omegaverse Girls!!!
I ABSOLUTELY LOVE Jillian West and her books!!! I’m so happy I already bought book two and now I have to buy the others for the Assurance Security series!! Not gonna lie Val kind of annoyed me at the beginning but she grew on me!! Her men are chef’s kisses!!! Holt annoys me some but I can let it slide. I already bought part two so I’m going to be reading that in between work phone calls!!!! DON’T TELL MY BOSS 😂😂😂😂
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Reviewed in the United States on September 30, 2025
C
Verified Purchase
Carmen Alicea
New York, US
★★★★★ 4
Baby bumps and bodyguards
Format: Kindle
Dark, emotional, and unexpectedly tender, Not Ready is an omegaverse romance that delivers found family feels, fierce protectiveness, and a very pregnant heroine who refuses to break. Vale’s on the run from a stalker, but lands in the arms of three private security alphas, cue the swoony tension, fake marriage twist, and slow-burn heat. It’s a little gritty, a little soft, and a whole lot addictive. If you love protective alphas, high stakes, and heroines with quiet strength, this one’s a must-read.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 18, 2025

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