SKU: 32236408624

Accuris qMax One-Step RT-qPCR Kit - BULK

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Description

Accuris qMax One-Step RT-qPCR Kit - BULKAccuris qMax One Step RT qPCR Kit BULK Accuris qMAX One Step Kits allow for highly sensitive real time RT qPCR assays to be performed directly from RNA templates. Workflows are simplified with optimized formulations of ready to use 2X qPCR master mix and 20X reverse transcriptase. Optimized buffer includes powerful RNase inhibitors and an extremely thermostable MMLV derived reverse transcriptase enables robust first strand cDNA synthesis. Accuris Hot

Accuris qMax One-Step RT-qPCR Kit - BULK

    Accuris qMAX™ One Step Kits allow for highly sensitive real time RT-qPCR assays to be performed directly from RNA templates.  Workflows are simplified with optimized formulations of ready-to-use 2X qPCR master mix and 20X reverse transcriptase.

    Optimized buffer includes powerful RNase inhibitors and an extremely thermostable MMLV-derived reverse transcriptase enables robust first strand cDNA synthesis.  Accuris Hot Start Taq uses an antibody mediated hot start mechanism allowing for sample preparation at room temperature.   Only after an initial incubation at 95C will the Taq become active, so non-specific amplification is greatly reduced.  An inert blue dye is included in the Taq master mix to help simplify pipetting and reduce errors.

    Three versions of our One Step qPCR kits are available:

    qMAX Green One Step kits incorporate our proprietary intercalating dye which exhibits higher fluorescent and lower PCR inhibition than other popular dyes such as SYBR.

    qMAX Probe One Step kits are optimized for use with popular TaqMan, Scorpions, and molecular beacon probes.

    qMAX Probe One Step Multiplex kits are specifically developed and optimized for efficient probe-based detection of multiple targets in a single reaction well.  The Multiplex formulation is comprised of a 20x reverse transcriptase and 2x PCR Mix preparation ideally suited for complex RNA samples including low-copy number viral RNA commonly used in the clinical and research laboratory.  qMAX Probe One Step Multiplex kits have been designed to overcome the many challenges of multiplex RT-PCR, by addressing important factors such as the balance between magnesium chloride and deoxynucleotide concentrations, the relative Taq Polymerase and reverse transcriptase concentration, and the ionic conditions of the core reaction buffer.

    All Accuris One-Step Kits are compatible with standard and fast cycling protocols and provide increased sensitivity, speed and reproducibility for a broad range of samples and targets.  The polymerase mix is available with different levels of ROX reference dye for compatibility with all qPCR instruments.

    • RNA to cDNA to qPCR, in one tube

    • High purity enzyme formulation for enhanced stability and performance

    • Accuris Hot-Start Taq allows for preparation at room-temperature

    • Blue dye facilitates pipetting and visualization in plates

    • Available for green fluorescence or probe detection

    • Multiplex formulation available for multiple target amplification

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    SKU: 32236408624

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    Bryan
    Chelsea, US
    ★★★★★ 4
    Fine Collection
    Format: Paperback
    A fine and accessible collection of poems that capture its time period and themes effectively. These poems are not for the faint of heart and will require some digging to fully enjoy but for what they are, Brooks is a genius. If you can't read them all please do read Sadie and Maud.
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    Reviewed in the United States on January 23, 2020
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    PA Book Lover
    Pawtucket, US
    ★★★★★ 5
    Beautiful and interesting poems
    Format: Paperback
    I came across this poet at a library book sale, and ordered this book to read more from this African-American writer. Excellent reflections of life and its difficulties and joys.
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    Reviewed in the United States on May 9, 2014
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    Paul Hancock
    Boise, US
    ★★★★★ 5
    Love it!
    Format: Paperback
    Love it!
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    Reviewed in the United States on April 10, 2023
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    Poet in the Desert
    Waukegan, US
    ★★★★★ 5
    Loved it!
    Format: Paperback
    I gave this book to an18 year old poet and she memorized several of the poems immediately. She loved the book and is interested in buying another collection by Brooks
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    Reviewed in the United States on July 17, 2013
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    lilygls
    Alexandria, US
    ★★★★★ 5
    Love The Poems
    Format: Paperback
    I love Ms. Brooks' poems. It's a great book of her work.
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    Reviewed in the United States on October 24, 2017

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