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Description
Rat CAT ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 50 ng/mL). Then dilute to the following concentrations: 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.125 ng/mL, 1.5625 ng/mL, 0.78125 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 50ng/mL standard working solution into the first EP tube and mix thoroughly to make a 25ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Catalase (CAT) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Catalase (CAT) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Catalase ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Catalase is a common enzyme found in almost all organisms exposed to oxygen, such as bacteria, plants, and animals, that catalyzes the decomposition of hydrogen peroxide into water and oxygen. It is a very important enzyme that protects cells from oxidative damage caused by reactive oxygen species (ROS). Catalase has one of the highest turnover rates of all enzymes; a single catalase molecule can convert millions of hydrogen peroxide molecules per second into water and oxygen. Catalase is a tetramer composed of four polypeptide chains, each with over 500 amino acids. It contains four iron-containing heme groups that enable the enzyme to react with hydrogen peroxide. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.78-50 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates and other biological fluids |
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4.6 ★★★★★
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Product Reviews
★★★★★ 5
Reliable Sun Protection for Active Days!
Size: 3 Fl Oz (Pack of 1), Style: SPF 50
Coppertone SPORT SPF 50 is my go-to sunscreen for outdoor activities. It’s lightweight, non-greasy, and stays on even when I’m sweating or swimming. The broad-spectrum formula protects against both UVA and UVB rays, giving me peace of mind during long hours in the sun.
The 3 fl oz size is perfect for carrying in a gym bag, backpack, or beach tote, making it easy to reapply throughout the day. It absorbs quickly and doesn’t leave a sticky residue, which is great for sports or active adventures.
Highly recommend for anyone who wants effective, durable sun protection without sacrificing comfort or mobility!
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Reviewed in the United States on November 21, 2025
★★★★★ 4
IT does protect from the sun.
Size: 5 Fl Oz (Pack of 1), Style: SPF 50
It protects from the sun without a doubt and lasts well. The downsides are that it leaves a greasy and "white" residue that no matter how much you rub it in.....it stays there. I use it when I am working outside and not planning on going anywhere anytime soon.
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Reviewed in the United States on May 20, 2026
★★★★★ 5
Fabulous
Size: 7 Fl Oz (Pack of 1), Style: SPF 50
Fabulous. Nice size bottle.
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Reviewed in the United States on May 24, 2026
★★★★★ 5
Great Sunscreen
Size: 7 Fl Oz (Pack of 1), Style: SPF 50
I cannot sing enough praises for Coppertone SPORT Sunscreen SPF 50 Lotion! This sunscreen is an absolute game-changer for anyone who loves outdoor activities. Here's why I think it deserves five stars:
Effective Protection: Whether I'm hiking, swimming, or playing beach volleyball, I trust Coppertone SPORT to keep my skin shielded from harmful UV rays. The SPF 50 protection gives me the confidence to enjoy the sun without worrying about sunburn.
Water Resistance: One of the standout features of this sunscreen is its water-resistant formula. It stays put even during sweaty workouts or dips in the pool, ensuring continuous protection throughout all my outdoor adventures.
Non-Greasy Formula: Unlike some other sunscreens that leave a greasy residue, Coppertone SPORT absorbs quickly into the skin, leaving it feeling smooth and hydrated without any stickiness. It's perfect for those who dislike the heavy feeling of traditional sunscreens.
Long-Lasting: I appreciate how long-lasting this sunscreen is. I can apply it in the morning before heading out for a day of activities, and it provides reliable protection for hours on end, saving me the hassle of frequent reapplication.
Suitable for Active Lifestyles: As someone with an active lifestyle, I need a sunscreen that can keep up with me. Coppertone SPORT ticks all the boxes with its durable and sweat-resistant formula, allowing me to focus on enjoying my time outdoors without constantly worrying about sun protection.
Overall, Coppertone SPORT Sunscreen SPF 50 Lotion has become a staple in my outdoor gear arsenal. It's dependable, effective, and comfortable to wear, making it a must-have for anyone who loves to stay active under the sun. I highly recommend it to fellow outdoor enthusiasts!
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Reviewed in the United States on April 20, 2024
★★★★★ 5
great
Size: 7 Fl Oz (Pack of 1), Style: SPF 50
works great on pale skin! doesn’t leave white residue
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Reviewed in the United States on June 3, 2026