Pay in installments of $46.03 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Jul 21 - Jul 26
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Rat MuRF1 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Muscle Ring-Finger Protein-1 (MuRF1). After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Muscle Ring-Finger Protein-1 (MuRF1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Muscle Ring-Finger Protein-1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | The E3 ubiquitin-protein ligase TRIM63, also known as "MuRF1," is encoded by the TRIM63 gene. This gene encodes a member of the RING zinc finger protein family, found in striated muscle and iris. The gene product is localized to the Z-line and M-line lattices of myofibers, where the N-terminal and C-terminal regions of titin, respectively, bind to the myofiber. In vitro binding studies have shown that this protein also directly binds to titin near the region containing kinase activity. Another member of this protein family binds to microtubules. Because these family members can form heterodimers, this suggests that these proteins may serve as a link between myosin kinase and microtubule-dependent signaling pathways in muscle. MuRF1 is the most commonly used name in the literature, standing for "muscle RING Finger 1." Structurally, there are two closely related MuRFs, MuRF2 and MuRF3. These also have the TRIM code. MuRF2 is TRIM55, and MuRF3 is TRIM54. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.6 ★★★★★
Based on 27 reviews
Sort
Product Reviews
★★★★★ 5
Single greatest thing I’ve ever bought for my dogs
Color: Charcoal, Size: 35" x 23"x 3.5", Color: Charcoal, Size: 35" x 23"x 3.5"
Few years ago we got our dog a dog. It was a bad idea. Our first dog is incredibly smart. He’d much rather hang out with humans, sitting in his own chair at the table while we play board games and chat than chase a ball. He uses talking buttons and picked it up quickly. Even as a little puppy he was kind of serious and when we’d take him to the dog park he’d mostly just run around barking at other dogs who were having too much fun.
Then came his brother who turned out to be the absolute opposite in every way. A silly, goofy, snuggly, floppy little muppet of a dog. Highly empathetic and Looooves to make people laugh. Our other dog mostly wanted nothing to do with him, barely tolerating him at the best of times. We thought, surely he’ll learn to love his brother. But 3 years later… nope.
Our first dog loves a good pillow so I thought this might make a good gift. I had no idea just HOW good. For some reason ever since our dog opened this gift he’s been obsessed. But so has our other dog. We initially were worried it would become one more thing for them to fight over BUT we were wrong. The dogs have been getting a long SO well! As long as it’s on this pillow, our first dog allows our second dog to come close enough to touch him.
They sleep next to each other, they wrestle, there’s no growling or grumping and our first dog will come over and lay next to his brother if he’s laying on this.
We’ve started calling it The Peace Pillow.
Anyway, I could have just said “it’s great” but it might actually be magic. Your experience may vary.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 12, 2026
★★★★★ 5
Super soft, cozy, and great quality Dog Bed
Size: Small 24"x18", Color: White, Size: Small 24"x18", Color: White
This pet mat exceeded my expectations. The material is incredibly soft and plush, and you can tell right away that it’s made with comfort in mind. My pet laid on it immediately, which is always the best sign. It provides just the right amount of cushioning without being too thick or heavy.
The quality is excellent — the stitching feels sturdy, the fabric doesn’t shed, and it holds its shape well with regular use. It’s lightweight and easy to move around, making it perfect for use in crates, on the floor, or even on furniture for extra protection. Cleaning is simple, and it still looks great after use.
Overall, this mat offers great durability, ease of use, and excellent value for money. It’s stylish, practical, and extremely comfortable for pets. I would absolutely recommend this to anyone looking for a cozy, high-quality mat for their dog or cat 🐾
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 24, 2025
★★★★★ 5
Cushion for the pooch’n
Size: Small 24"x18", Color: Brown
I’ve been using the ULIGOTA Dog Bed Crate Pad Plush Soft Crate Bed in my dog’s crate for daily resting and overnight sleep.
Right out of the package, it’s clearly designed for comfort first. The plush top feels soft and inviting, and it immediately makes the crate look more like a cozy sleeping space rather than a hard plastic tray. Once fluffed up, it provides a decent layer of cushioning that’s noticeably more comfortable than a bare crate floor or thin mat.
In terms of fit, it sits well inside standard crate sizes and doesn’t bunch up too badly once settled. The anti-slip bottom helps keep it in place during normal movement, which is especially helpful for dogs that reposition a lot while sleeping.
Comfort-wise, it works well for small to medium dogs that just need light padding. It’s warm and soft enough for everyday use, and most dogs seem to settle into it quickly. However, it’s not a high-density or orthopedic-style bed, so it doesn’t offer much support for dogs that need joint relief or extra structure.
Durability is average. It holds up fine under normal sleeping use, but it’s not ideal for heavy chewers or dogs that dig aggressively. Like most plush crate pads, it’s more of a comfort layer than a rugged long-term solution.
Pros: very soft plush surface, comfortable for everyday crate use, easy fit in standard crates, non-slip backing helps stability
Cons: not chew-resistant, limited support for heavier or senior dogs, cushioning is on the thinner side
Overall: a solid, budget-friendly crate pad that improves comfort and warmth significantly over a bare crate, but best suited for calm sleepers rather than heavy chewers or dogs needing orthopedic support.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 19, 2026
★★★★★ 5
Soft dog bed perfect for small breeds
Size: Medium 30"x20", Color: White
This dog bed is very soft and fit perfectly in my dogs crate. I like that it came vacuum sealed and was really easy to open and leave out to expand. The material is durable and it is perfect thickness for a small breed dog. My Maltese puppy loves it and has been sleeping so well on it, she is very comfortable. The material is durable and easily washable, I already had to run it through the washing machine. Well worth the price and easy to fit in a crate or small space.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 23, 2026
★★★★★ 4
fluffy
Size: Small 24"x18", Color: White
good bed and fairly durable for chewers but when torn, stuffing everywhere. also useful as a toy for smaller dogs.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 5, 2026