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Description
Human ADAM33 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment:
1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against A disintegrin and metalloprotease 33 (ADAM33). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and then to yellow by acid. The intensity of the color is positively correlated with the amount of A disintegrin and metalloprotease 33 (ADAM33) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human A Disintegrin And Metalloprotease 33 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | disintegrin and metalloproteinase 33 (ADAM33) is an enzyme encoded by the ADAM33 gene. It is a member of the ADAM (disintegrin and metalloproteinase domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrin and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This protein is a type I transmembrane protein associated with asthma and bronchial hyperresponsiveness. Alternative splicing of this gene results in two transcript variants encoding different isoforms. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.9 ★★★★★
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Product Reviews
★★★★★ 5
Works well
Size: 2.3 Pound (Pack of 1), Style: Sugar Free 180 count
My doctor encouraged my use of this product to lower my cholesterol. Okay, months later, no difference with cholesterol, but the produce makes elimination very very clean and I like that, so I still use this product.
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Reviewed in the United States on May 28, 2026
★★★★★ 5
Helps me a lot
Size: 2.3 Pound (Pack of 1), Style: Sugar Free 180 count
Psyllium husk is a very necessary supplement and increases my daily fiber intake. It helps me use the bathroom consistently as it makes my bowel movements more regular. The taste of this is similar to orange juice and does not have a lot of sugar. I like putting this in my water every day. It is very easy to use and just dissolves in liquid. This large container will last me for over a month so it’s a great value for the money.
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Reviewed in the United States on May 10, 2026
★★★★★ 5
“My Everyday Essential: Nourishment for Skin, Health & Wellness”
Size: 32 Fl Oz (Pack of 1)
I’ve been using Viva Naturals pure coconut oil every day, and it has truly transformed my routine. I love all things natural, and I use this oil in so many ways that it has become indispensable. I take three baths a day and then rub it all over my body; my skin has never felt so soft and hydrated.
In the mornings, I take a big tablespoon on an empty stomach, and sometimes I mix it into my smoothies with flaxseed and almond milk. I feel lighter, more energetic, and I know I’m taking care of my health from the inside out. For me, there’s nothing like pure coconut oil: it’s versatile, natural, and truly wonderful. I highly recommend it to anyone looking for a healthy product for their skin and daily wellness.H
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Reviewed in the United States on March 29, 2026
★★★★★ 5
Ingredient for Bulletproof coffee, good stuff!
Size: 32 Fl Oz (Pack of 1)
Bulletproof Coffee. Dave Asprey. Look it up. This isn't his MCT oil, which I'm sure is spectacularly better, but I'll use the Pareto principle (80-20 Rule) and settle for a much less expensive version for a slightly lowered benefit. Perhaps I'll upgrade when I'm more of a genius and fabulously wealthy... ;)
So, I put this in my morning protein smoothie, which I make cold with pre-brewed coffee that is chilled...
Wait, what is MCT Oil? Medium Chain Triglyceride Oil. Basically, it is specially refined coconut oil, where they isolate only the MCT portion of the oil. I'll let you look up why medium chain triglycerides are fantastic. But they ARE fantastic! Worth the read.
So what does it do? Dave would say it is brain- fuel. Since our brains are made up mostly of fat, our brain naturally wants (healthy) fats for food. And MCT is some of the best possible oil/fat for that purpose. Plus, MCT has a benefit of being self-preserving, unlike many oils which go rancid (or oxidize) rapidly. Rancid oils are actually sort of toxic, in that they start to have damaging effects instead of beneficial ones. Kind of like the difference between eating a nice juicy steak...or 3 day old roadkill. And yes, I think that's an accurate analogy.
How did it work for me? Well, true enough, with the coffee and protein, I do have clear-headed mornings, and I feel energized (provided I get enough sleep the night before!). I have noticed that I don't seem to be hungry as early, and I attribute that to the "full" or satiation effect of the oil. Fats satisfy our hunger needs, because they are valuable to the body. (Even bad fats satisfy, but it's not good for us).
Will I buy this again? Absolutely. Probably have a bottle of this in my pantry forever now. And I also have virgin organic cold-pressed (I think) coconut oil as well, which I use for cooking, eating (straight), and use on my skin. MCT Oil is for eating (drinking) in my smoothie, as brain fuel.
I've never used Viva Labs before, but happy with them, and will be alert if they have other products I want. The bottle is high quality, and the cap stays tightly closed. Very pleased overall, everything is positive. Glad I chose them for this, and glad this does what I got it for!
Taste is faint, not very strong anything. Texture is like a thin mineral oil (viscosity if you want to be technical).
As a side note, if I needed a lubricant for the bedroom, I would grab this before the unrefined coconut oil. Not sure how it works with condoms, have not used this with them, so if you are relying on them and this, do your homework on compatibility...might eat some kinds or others.
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Reviewed in the United States on March 3, 2016
★★★★★ 5
it checks every box for clean, high-quality nutrition.
Size: 32 Fl Oz (Pack of 1)
The oil itself is completely flavorless, which makes it perfect for adding to my morning coffee, smoothies, or even drizzling over meals. It blends smoothly and gives me a steady, sustained boost without any jitters or crashes. The 32 fl oz size is also a great value and lasts a long time.
If you’re looking for a pure, reliable MCT oil to support focus, energy, or a keto lifestyle, this one is a winner. I Highly recommended!
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Reviewed in the United States on November 30, 2025