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Description
Mouse IgG1 Onestep ELISA KitProduct Specification Antigen IgG1 Immunogen Recombinant Protein Antibody Type Recombinant mAb Reactivity Ms Purification Protein A Stability & Storage 12 months from date of receipt reconstitution, 2 to 8'C as supplied. Kit Precision Intra assay: 4. 0% Inter assay: 5. 6% Sample type Cell culture supernatant; Serum; Plasma Assay type Sandwich (quantitative) Sensitivity 0. 173 ng mL Range 0. 47 ng mL 30 ng mL Recovery Cell culture supernatant: 83%
Product Specification
| Antigen | IgG1 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Ms |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 4.0%； Inter-assay: 5.6% |
| Sample type | Cell culture supernatant; Serum; Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 0.173 ng/mL |
| Range | 0.47 ng/mL – 30 ng/mL |
| Recovery | Cell culture supernatant: 83% Serum: 104% Plasma: 102% |
| Assay time | 60 minutes |
| Species reactivity | Ms |
Background
Mouse IgG1 is a monomeric immunoglobulin gamma subclass that constitutes a significant proportion of serum antibodies. It is particularly prominent in responses to protein antigens and is often associated with Th2-type T-cell responses. A key functional characteristic of IgG1 is its relatively low affinity for activating Fc gamma receptors (FcγRs) on immune cells like macrophages and neutrophils. Consequently, it is generally poor at triggering effector functions such as antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC). However, it has a high affinity for the inhibitory FcγRIIB receptor, which can dampen immune activation. IgG1 is therefore often considered an antibody isotype that primarily functions to neutralize pathogens and toxins without vigorously activating inflammatory pathways.
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