Pay in installments of $46.03 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Aug 24 - Aug 29
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Human CD40L ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Cluster of Differentiation 40 Ligand (CD40L) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Cluster of Differentiation 40 Ligand (CD40L) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Cluster Of Differentiation 40 Ligand ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | CD40L is a protein primarily expressed on activated T cells and a member of the TNF superfamily. It binds to CD40 (protein) on antigen-presenting cells (APCs), leading to a variety of effects depending on the target cell type. CD40L has three binding partners: CD40, α5β1 integrin, and αIIbβ3. On TFH cells, it promotes B cell maturation and function by binding to CD40 on the surface of B cells, thereby facilitating cell-to-cell communication. Defects in this gene result in an inability to undergo immunoglobulin class switching and are associated with hyper-IgM syndrome. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.8 ★★★★★
Based on 28 reviews
Sort
Product Reviews
★★★★★ 5
GREAT CONCEPT AND DESIGN
I bought these Feminine Balance Gummies because I was intrigued by the approach that was utilized. For me, I have been involved with lactobacillus formulas and probiotics with incorporating yogurt (the really healthy kind not the ones with a lot of advertising) to deal with yeast overgrowth. Typically I am experiencing a problem because I am consuming too much sugary foods (causing the body's resources to be depleted) or because I have taken a course of antibotics which killed both the good and bad bacteria in the body and now needs replenished.
So, my first impression was the intrigued by the use of Pineapple as a part of the formulation, which is unusual but a good choice nonetheless because it is very beneficial for digestion and also for pain reduction. I liked the taste of the gummy, it tasted like Pineapple candy. The inclusion of Vitamin C seemed a bit of a stretch and is included to enhance the immune function. There are lactobacillus strains but the manufacturer has kept the strains used as a proprietary secret - which I find a bit frustrating as a consumer. Whatever it is, there is a billion of them (is this per gummie?).
The manufacturer does support the use of vegan only products, it is also "clean" Halal, it is gluten free and allergen free. So, it appears they have carefully considered the inclusion of each ingredient for benefit.
So here is the real test, yes, I have had the desired outcome by improving my "lady garden" and this is a very viable product in my opinion. I would recommend it to others.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 30, 2026
★★★★★ 1
They are hard to chew
Gummies are old and hard, still tried them cause I want a fantastic kitty and it actually did the opposite. It’s been itchy and irritated with every that comes in contact with it. I never had a problem down there but now I do. It’s a no go for me.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 3, 2026
★★★★★ 5
Tasty, Easy‑to‑Take Gummies for Daily Feminine Balance Support
I picked these up because I wanted a simple, vegan option to support pH balance and overall feminine wellness, and these gummies have been really easy to add into my routine. The Hawaiian pineapple flavor is surprisingly good—sweet without being artificial—and the texture is soft and easy to chew.
I like that they combine multiple areas of support in one: healthy odor, flora balance, and immune support. It makes them feel like a well‑rounded daily supplement instead of juggling multiple bottles. They’re also non‑GMO and vegan, which is a big plus if you’re trying to keep your routine clean.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 16, 2026
★★★★★ 4
Tastes Great, But More General Wellness Than Targeted Feminine Support
I tend to prefer gummies over capsules whenever possible, so these Yalei 4-in-1 Nuora Feminine Balance Gummies caught my attention as a potential replacement for my usual women’s probiotic.
The bottle contains 60 gummies (30 servings), with a formula centered around vitamin C, pineapple fruit powder, and Bacillus coagulans (1 billion CFU). The ingredient list is clean and clearly labeled, and I appreciated that it’s free from common allergens like soy, dairy, gluten, and tree nuts. The outer and inner tamper seals were intact. The manufacturing and expiration dates were also easy to read, which is something I always look for with supplements.
Taste-wise, these are genuinely enjoyable. The pineapple flavor is pleasant, not overly artificial, and there’s no lingering aftertaste. The texture is firm without being sticky, which makes them easy to take daily. Honestly, these fall into that category where it’s tempting to eat more than the serving size.
Where things became more nuanced for me was performance. I switched from a more targeted women’s probiotic that included specific Lactobacillus strains and additional D-mannose and cranberry support ingredients. Within about two weeks of making the swap, I experienced a UTI flare-up, which led me to go back to my previous formula.
That experience made the difference in formulation stand out. My prior supplement included strains commonly associated with vaginal microbiome support along with additional complementary ingredients, whereas this product uses a single Bacillus-based probiotic strain. From my experience, this felt more like a general wellness probiotic gummy rather than something formulated for more targeted feminine health concerns.
That doesn’t make it a bad product–it just depends on what you’re looking for. If your goal is a simple, good-tasting daily probiotic with light supportive ingredients, this fits that role well. If you’re specifically trying to maintain balance in more sensitive or recurring situations, you may want something more specialized.
The brand also includes details like GMP manufacturing claims and batch testing which adds a layer of reassurance, though it does appear to be a newer brand.
At its current lower price point, I do think it offers decent value for a general-use gummy. For me personally, though, I’ll be sticking with a more targeted formula moving forward.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 17, 2026
★★★★★ 5
Probiotic fruit snacks
Disclaimer: vine review of an item I requested free*
These taste great, just like fruit snacks. I've gotten a lot of gummies from this brand and haven't found a bad one yet. Pineapple isn't even a flavor I usually like, but these taste good. Somehow they're always sweet with no weird aftertaste without having added sugar. Not too sticky, it doesn't leave residue on your fingers, but they do sometimes stick together.
Mainly I got these to have some fruit snacks to eat, but the probiotics are a nice bonus and now I can pretend I'm being healthy when I eat my candy.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 4, 2026