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Description
Alexa Fluor® 647 Rat Anti-Mouse CD9 Antibody (S-R652)Product Specification Host Rat Antigen CD9 Synonyms CD9 antigen; Cd9 Location Cell membrane Accession P40240 Clone Number S R652 Antibody Type Rat mAb Isotype IgG2a,k Application FCM Reactivity Ms Positive Sample C57BL 6 mouse bone marrow Purification Protein G Concentration 0. 2 mg ml Conjugation Alexa Fluor 647 Physical Appearance Liquid Storage Buffer PBS, 1% BSA, 0. 3% Proclin 300 Stability & Storage 12 months from date of receipt reconstitution,
Product Specification
| Host | Rat |
| Antigen | CD9 |
| Synonyms | CD9 antigen; Cd9 |
| Location | Cell membrane |
| Accession | P40240 |
| Clone Number | S-R652 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse bone marrow |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Ms |
Background
CD9 is a 24-kDa tetraspanin membrane glycoprotein with four transmembrane domains and two extracellular loops that is widely expressed on hematopoietic and non-hematopoietic cells, where it organizes tetraspanin-enriched microdomains that integrate adhesion, signaling and trafficking by associating with integrins, EWI-2/F, and other tetraspanins to regulate cell adhesion, migration, fusion, exosome biogenesis/secretion, immune activation and tumor suppression, and its large extracellular loop is essential for sperm–egg fusion, while its presence in exosomes marks these vesicles for intercellular communication and its altered expression influences cancer progression, viral entry and stem-cell pluripotency.
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