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Description
Human CDHE ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant and centrifuge at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Urine: Tissue homogenate: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a cadherin, epithelial (CDHE) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of cadherin, epithelial (CDHE) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Cadherin, Epithelial ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Epithelial cadherin (CDHE) is a member of the classic subfamily of the mucin family and the most important cadherin. Predominantly present on epithelial cell membranes, it is a crucial molecule for maintaining epithelial cell morphology, structural integrity, and polarity. It maintains close contact between tumor cells, making them difficult to dissociate. Its sustained expression and functional activity are essential for maintaining close contact between cells in epithelial tissues. Without it, many other cell-adhesion and cell-junction proteins expressed in epithelial cells cannot support intercellular adhesion. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, urine, tissue homogenates and other biological fluids |
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4.8 ★★★★★
Based on 12 reviews
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Product Reviews
★★★★★ 5
Great Accent Piece
Size: 48in, Color: 3tier
Industrial looking with wide shelf space. We were looking for a functional accent piece that we can also put things on. This new addition has been more than perfect. It provides a rustic feel and it has the industrial bars that holds the shelves which is the best.
Very sturdy, please make sure to assemble on studs this piece is heavy. It looks really good in our living area.
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Reviewed in the United States on June 12, 2025
★★★★★ 5
Good looking
Perfect. Easy install. Sturdy.
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Reviewed in the United States on August 31, 2025
★★★★★ 5
It takes skill to mount and not have the leaning tower of Pisa
Size: 36in, Color: 4-layer
First, the product is beautiful! I purchased this one for a smaller wall location but realized it was more massive and commanding within a room - That ended up being a good thing as I decided to put it in my office where my L shaped desk, (a Vernal executive desk), being quite massive, was successfully offset by drawing the eye up to the wall behind the desk where I had this mounted - In essence, the room was now properly balanced with the large desk - One should also know it takes some skill to mount to the wall - It should be in studs - You have to use a balance or bubble level to first get the vertical pipes parallel to each other - Also, make sure both vertical pipe supports are symmetrically matching each other from one side to the other - If you mount one upside down the wood shelves won’t lay level - We also turned the pipe supports so that the shelves would be equal distant - If I recall, if they are turned the other direction , you end up with shelves that are not equal distant with 2 shelves ending up closer to each other than the remaining shelves. The shelving is very high quality and I was very, very pleased with the end result and how the whole room was balanced with the large desk in a smaller office space.
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Reviewed in the United States on May 22, 2026
★★★★★ 5
Solid shelves that look good!
Size: 36in, Color: 3-layer, Size: 36in, Color: 3-layer
Got these as a focal piece to my basement bar (added some cheap stick on LED strip lights behind it).
I’m glad I ordered these! They are sturdy, shelves are solid wood, and the overall quality of the shelves is great. They were easy to install with provided hardware (although there isn’t much instruction in terms of mounting them, just putting them together), but it was simple enough to figure out with two people and a level (one holding, one making marks). Each pipe end has 4 screw holes, so expect to put 16 holes in your wall, but as shown in the photos the shelves can hold a good amount of weight. I recommend these for anyone looking to add the industrial look to your wall with a quality built shelf.
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Reviewed in the United States on August 7, 2023
★★★★★ 5
VERY sturdy and almost foolproof
Size: 36in, Color: 3-layer, Size: 36in, Color: 3-layer
This kit is made of real wooden planks, and metal pipes and fittings. Installation is simple- assemble each end of the kit, hold it up to the wall using a magnetic level, mark the four holes for each mounting piece, then repeat with the other side. You'll need a tape measure to make sure that the ends aren't too close together or too far apart, and measure from the ceiling or floor to ensure a level surface. You will want a second set of hands for most of the install process.
This kit comes with good molly bolts and strong screws that match the finish of the metal hardware. Make sure that each side of the unit is oriented the same way, as I had to flip one side after neglecting to do so at first. Retaining brackets and wood screws are included for securing each shelf to the metal frame. A power drill makes this part very easy, and can be done mostly solo as the brackets can be pinched to clip onto the frame while you attach the screws.
Once installed, this unit is VERY sturdy. There is no play or wobbling when testing the attachment to the wall. If there is, tighten your screws and make sure that the fittings to the wall mounts are hand-tight. These shelves can handle heavy kitchen staples and books with no problem. It is a tier more expensive than most Ikea-style shelving, but that's worth it for the confidence that my dry goods are safely stored in a convenient place.
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Reviewed in the United States on July 10, 2023