Pay in installments of $46.03 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Aug 26 - Aug 31
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Human BECN1 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. 5. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. 6. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Beclin 1 (BECN1) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of Beclin 1 (BECN1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Beclin 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | Beclin-1 (BECN1) is a protein encoded by the BECN1 gene. Beclin-1 is the mammalian ortholog of yeast autophagy-related gene 6 (Atg6) and the nematode BEC-1. This protein interacts with Bcl-2, BCL2L2, GOPC, and MAP1LC3A, playing a key role in regulating autophagy and cell death. Schizophrenia is associated with low levels of Beclin-1 in the hippocampus of affected individuals, which leads to impaired autophagy and, in turn, increased neuronal cell death. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.1 ★★★★★
Based on 12 reviews
Sort
Product Reviews
★★★★★ 5
Still One of the Best Cleansing Oils I’ve Ever Used After Years of Repurchasing
Scent: Unscented, Size: 7.7 Fl Oz (Pack of 1)
I rarely stay loyal to skincare products for years because I’m always testing and trying new things, but DHC Deep Cleansing Oil is one of the very few products that has remained a permanent part of my routine. I’ve repurchased this multiple times over the years, and every time I try another cleansing oil, I eventually come back to this one because the performance is just consistently good.
The first thing that stands out is how effective it is at removing makeup. I wear waterproof mascara, eyeliner, sunscreen, and long-wear foundation regularly, and this breaks everything down incredibly fast without needing to aggressively rub my skin. I usually apply one pump onto dry hands and massage it onto my dry face for about 30 seconds. You can literally feel the makeup dissolving almost immediately, especially around the eye area where stubborn makeup usually takes extra effort to remove.
What I really appreciate is that it removes heavy makeup in one application. I don’t have to go back in multiple times or use separate eye makeup removers anymore. Even waterproof mascara that normally leaves residue around the lashes comes off cleanly. That alone saves me so much time at night when I’m exhausted and just want my skincare routine done quickly.
Once water is added, the oil transforms into a light milky texture that spreads evenly and rinses away very easily. A lot of cleansing oils claim to rinse clean but still leave behind a greasy film that makes me feel like I need another cleanser immediately afterward. This one actually rinses off properly with water and leaves my skin feeling clean, soft, and balanced instead of heavy or sticky.
Another reason I’ve continued using this for years is because it doesn’t make my skin feel stripped. My skin tends to get dehydrated easily if I use harsh cleansers, especially during colder months, but this leaves my face feeling comfortable and hydrated. The olive oil and vitamin E in the formula definitely help with keeping the skin soft after cleansing. My skin usually feels smoother and calmer afterward instead of tight and irritated.
I also haven’t experienced clogged pores from this, which surprised me because I was originally nervous about putting an oil cleanser on my face. Instead, it actually helps loosen buildup and sunscreen better than many foaming cleansers I’ve tried. My skin texture always looks cleaner and more refreshed after using it consistently.
The bottle also lasts a very long time because you honestly don’t need much product. One pump covers my entire face easily, including eye makeup removal. Even with regular use, the full-size bottle lasts me months, so I feel like the value is actually very good considering how little product is needed each time.
The included travel size is another nice bonus. I keep the mini bottle in my travel bag or purse because it’s convenient for trips and prevents me from having to transfer product into another container. It’s small but still lasts quite a while.
One more thing worth mentioning is the price. I’ve purchased this from different places over the years, and this listing is usually cheaper than buying directly from the official store. Whenever I see it on sale, I stock up because it’s one of those products I genuinely never want to run out of.
Overall, this is one of the most reliable cleansing oils I’ve used. It removes makeup quickly, rinses clean without residue, feels gentle on the skin, and lasts a long time. If you wear makeup regularly, use waterproof products, or simply want a cleansing oil that actually performs the way it promises to, this one is absolutely worth trying.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 15, 2026
★★★★★ 5
my holy grail
Scent: Unscented, Size: 6.7 Fl Oz (Pack of 1)
i’ve been repurchasing this for almost 8 years now. hands down this is a holy grail product for me and i have been recommending it to everyone. it takes my makeup off great and it leaves my skin feeling so smooth and soft. its very lightweight and it is very easy to use. i use this for about 10-20 minutes before i use a water based cleanser to take it off. it got rid of little bumbs i have and i genuinely do feel blackheads and dead skin coming off as i use it. this made my skin do a 180 and i will def be using this until the day i die. i love this and recommend it so much.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 5, 2026
★★★★★ 4
Reliable choice
Scent: Unscented, Size: 6.7 Fl Oz (Pack of 1)
Product I have been purchasing for almost a decade. Excellent at getting out black heads around the nose and mouth. However, if you do not thoroughly double cleanse after, you will be sure to experience a break out. It has a mild scent that doesn’t bother me, typically I get headaches from too strong of a scent. Overall, it’s a gentle way to cleanse and protect your skin health.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on October 20, 2025
★★★★★ 5
Holy grail of cleansing oil!
Scent: Unscented, Size: 6.7 Fl Oz (Pack of 1)
I'm very experienced with skincare, and this is hands down the best cleansing oil I've come across. I use this as part of a double cleansing routine, and it is so effective in removing makeup, sunscreen, and difficult mascara I no longer need to use makeup remover balm as a first step. This is now a permanent part of my skincare routine!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 17, 2026
★★★★★ 5
Truly does clean even mascara and eyeliner and there is no oily residue - just a clean face!
Scent: Unscented, Size: 7.7 Fl Oz (Pack of 1)
I won’t be without this face cleansing oil! It works like magic to remove every trace of foundation, eye liner, mascara, eyeshadow and leaves my face feeling hydrated but not greasy or filmy. It rinses off easily - I have used it for 10+ years . It never causes breakouts nor does it leave residue. I am 75 years old.,
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 24, 2026