SKU: 12613136433

Anti-FITC Acceptor Beads

Sale price$303.75 Regular price$337.50
Save 10%

Pay in installments of $84.38 with ShopPay, AfterPay and Klarna

Shipping Estimate
USA
  • USA
  • CAN

Ships within 48 hours · Estimated delivery Aug 24 - Aug 29

Promo Codes Available:

For Your Every Summer RSVP, with Code: SUMMER15

Description

Anti-FITC Acceptor BeadsProduct Specification Stability & Storage Store in a dark place at 28; product shelf life is 12 months. Background Homogeneous Immuno Chemiluminescence Assay (HICA) is a homogeneous immunoassay based on energy transfer between donor beads and acceptor beads at close proximity, resulting in luminescence. Donor beads recognize Protein 1 (Tag1 label), while Acceptor beads recognize Protein 2 (Tag2 label). When Protein 1 binds to Protein 2, the distance

Product Specification


Stability & Storage

Store in a dark place at 2~8℃; product shelf life is 12 months.

Background

Homogeneous Immuno Chemiluminescence Assay (HICA) is a homogeneous immunoassay based on energy transfer between donor beads and acceptor beads at close proximity, resulting in luminescence.

Donor beads recognize Protein 1 (Tag1 label), while Acceptor beads recognize Protein 2 (Tag2 label). When Protein 1 binds to Protein 2, the distance between the beads becomes less than 200nm. Upon excitation at 680nm, the donor beads generate singlet oxygen, which diffuses to the acceptor beads. The acceptor beads then undergo a redox reaction, emitting light at 615nm. The signal intensity is directly proportional to the strength of the protein interaction.

This product offers a simple, wash-free operation process, with high speed and sensitivity, enabling the detection of weak interactions.

Components

Specification

Volume

250 μg

50 μL

5 mg

1 mL

25 mg

1 mL x 5


Protocol

[Required Reagents]

Name

Catalog No.

Anti-FITC Acceptor Beads UA086101
Streptavidin Donor Beads UA086104
Universal Buffer 1 UA086113


[Detection Protocol for Reference]

Protocol

Protocol 1 (Rapid detection at 37°C)

Protocol 2 (Room temperature detection)

Step 1:

4μL Tag1-M1 +4μL Tag2-M2+ 6μL Acceptor Beads,Protect from light / Green light

4μL Tag1-M1 +4μL Tag2-M2+ 6μL Acceptor Beads,Protect from light / Green light

Incubation

Shake incubate at 37°C for 20 minutes,Protect from light / Green light

Incubate at room temperature for 60 minutes,Protect from light / Green light

Step 2:

Add 6μL Donor Beads,Protect from light / Green light

Add 6μL Donor Beads,Protect from light / Green light

Incubation

Shake incubate at 37°C for 10 minutes,Protect from light / Green light

Incubate at room temperature for 30 minutes,Protect from light / Green light

Reading

Instrument reading

Instrument reading


[Performance Validation]

Sample Preparation:

Dilute FITC-BSA-Biotin to 6.6 μg/mL (100 nM) as stock solution using Universal Buffer 1, then perform serial dilutions according to the following scheme:

No.

Final Concentration (nM)

Universal Buffer 1

Volume (μL)

High Conc. Addition

Volume (μL)

C12

1.0E+01

210

90 μL Stock Solution

C11

3.0E+00

210

90 μL C12

C10

1.0E+00

180

90 μL C11

C9

3.0E-01

210

90 μL C10

C8

1.0E-01

180

90 μL C9

C7

3.0E-02

210

90 μL C8

C6

1.0E-02

180

90 μL C7

C5

3.0E-03

210

90 μL C6

C4

1.0E-03

180

90 μL C5

C3

3.0E-04

210

90 μL C4

C2

1.0E-04

180

90 μL C3

C1

0

180

/


Detection Reagent Preparation:

Name

Preparation Concentration

Diluent

Anti-FITC Acceptor Beads

25 μg/mL

Universal Buffer 1

Streptavidin Donor Beads

25 μg/mL

Universal Buffer 1


Results for 37°C Incubation Mode:

Maximum Signal: 2779107

Minimum Signal: 1906

EC50= 0.290 nM

Results for Room Temperature Incubation Mode:

Maximum Signal: 1095538

Minimum Signal: 754

EC50= 0.334 nM

Guidelines

1. This experiment is light-sensitive; ensure all procedures (preparation, loading, and incubation) are performed in a dark environment. Recommended to operate under green light (illuminance below 100 LUX). 2. This product is compatible with multi-functional microplate readers equipped with Alpha detection modules. 3. Vortex thoroughly before use. Alternatively, briefly centrifuge (2000×g, 5–10 seconds) to ensure complete sample retrieval. 4. It is recommended to use the accompanying dilution buffer for reagent preparation and sample dilution. If additional components are required, they can be directly added to this buffer. 5. To ensure comparability of experimental data across different batches, strictly control incubation temperature and duration. 6. Avoid bubble formation during sample loading.
Shipping Notes
  • Free Standard Shipping on $100+ Orders to the USA.
  • Except Preorder products are shipped in 48 hours.
  • Delivery to the USA:
  1. Standard Shipping : 3-10 business days
  • If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
  • We offer a 30-day return/exchange service after receiving.
  • Final sale items are not eligible for returns or exchanges.
  • To process your return/exchange, please contact us at [email protected]
  • Please click here for more details>>> Return & Exchange Policy
SKU: 12613136433

Discover Niche Categories That Outsell

Top-Converting Item to Boost Your Average Order

4.4 ★★★★★
Based on 25 reviews
Sort
Highest Rating
Newest First
Oldest First
Product Reviews
P
Verified Purchase
Pam Coon
Fort Morgan, US
★★★★★ 4
Thin and cheap
Package Size Name: XXL, Size: 150 sheet (Pack of 12)
Very thin, cheap and just not what I was expecting based on the great reviews and paid a little more too. Meh.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 1, 2026
B
Verified Purchase
Bella Andrea
Cuba, US
★★★★★ 5
Great option. For less clean up! these are a must
Style: Modern, Size: 90 Count (Pack of 1)
Inexpensive, sturdy, perfect instead of heavy dishes. Good versatility and size. Thick and they don’t crumple when a full supper is on them.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 29, 2026
S
Verified Purchase
SHANA DANIELS GARDNER
Grantham, US
★★★★★ 5
Dependable Quality, Great Value - A Household Essential!
Style: Modern, Size: 90 Count (Pack of 1)
These Dixie Medium Paper Plates (8.5 Inch, 90 Count) are our absolute go-to, and we always make sure to keep them stocked in our house! The quality is consistently excellent – they truly are 2X stronger, microwave-safe, soak-proof, and cut-resistant, which means no more leaky messes or flimsy plates. You get fantastic reliability without breaking the bank. For everyday meals, parties, or any occasion where you want convenience without sacrificing quality, these are perfect. A great product at a good price point. Highly recommend!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 13, 2026
W
Verified Purchase
Winter
Fort Morgan, US
★★★★★ 5
Washable quality paper plates
Style: 10 Inch, Size: 150 Count (Pack of 1)
We've used these paper plates for years for all kinds of things and find them to be really sturdy and washable. With a pleasant design that fits any decor, they are wonderful for everyday use. I really like the fact that the top surface can be lightly washed and the plates reused. They come in a package of 150 and are really good value for the money.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 25, 2026
M
Verified Purchase
Millie
Chelsea, US
★★★★★ 5
Great paper plates
Style: 10 Inch, Size: 150 Count (Pack of 1)
My favorite paper plates. Beautiful colors, sturdy and great price and let’s not forget the size. They are a great size that fits a lot of food.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 22, 2026

recommand products