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Ships within 48 hours · Estimated delivery Jul 25 - Jul 30
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Description
Human TP53 antibody (p53 antibody) ELISA KitThe Human TP53 antibody (p53 antibody) ELISA Kit measures TP53 Ab in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antigen Sandwich ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP detection antigen, then it
The Human TP53 antibody (p53 antibody) ELISA Kit measures TP53-Ab in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antigen-Sandwich ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP-detection antigen, then it binds with TP53-Ab bound to precoated antigen. Wash unbound components and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of TP53-Ab in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
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Exchange/Return Notes
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