FT4 (Free Thyroxine) ELISA Kit

FT4 (Free Thyroxine) ELISA KitThe FT4 (Free Thyroxine) ELISA Kit measures fT4 in Universal Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with anti fT4 antibody. Sample or standard completely mixes with fixed amount of biotin labeled fT4 working solution and also add into each well. fT4 in the sample or standard competes with

The FT4 (Free Thyroxine) ELISA Kit measures fT4 in Universal Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with anti fT4 antibody. Sample or standard completely mixes with fixed amount of biotin-labeled fT4 working solution and also add into each well. fT4 in the sample or standard competes with biotin-labeled fT4 to bind with antibody precoated on the microplate. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of fT4 in the sample. The concentration of the target substance is inversely proportional to the OD450 value.