Human TXNIP (Thioredoxin-interacting protein) ELISA Kit

Human TXNIP (Thioredoxin-interacting protein) ELISA KitThe Human TXNIP (Thioredoxin interacting protein) ELISA Kit measures TXNIP in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti TXNIP antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add

The Human TXNIP (Thioredoxin-interacting protein) ELISA Kit measures TXNIP in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti TXNIP antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with TXNIP bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of TXNIP in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.