SKU: 90264988808

Human NR1H4 (Bile acid receptor) ELISA Kit

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Description

Human NR1H4 (Bile acid receptor) ELISA KitThe Human NR1H4 (Bile acid receptor) ELISA Kit measures NR1H4 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti NR1H4 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection

The Human NR1H4 (Bile acid receptor) ELISA Kit measures NR1H4 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti NR1H4 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with NR1H4 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of NR1H4 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 90264988808

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4.7 ★★★★★
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Verified Purchase
Kendra
New York, US
★★★★★ 5
Love this book!
Format: Hardcover
I love this book and so does my 3 year old!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 1, 2023
L
LAS Reviewer
Phoenix, US
★★★★★ 5
Good Reading
Format: Paperback
The third book we chose to read for our summer reading program was Not That Pet! I am a nanny of a six-year-old girl going into first grade. It’s fun seeing a child’s eyes light up when a book or story engages their imagination. This story was another hit. This was an entertaining read! This is a rhyming read-aloud story that had us both giggling from the beginning of the story to the end. The main character, Mabel, is allowed to pick the family pet. She is determined to find the perfect pet. Her choices had us groaning, ‘oh nooo’ while shaking our heads and laughing out loud. We couldn’t read fast enough to see Mabel’s next choice. Her final decision was unpredictable. We couldn’t believe the final choice was a…. well, you’ll just have to read the story yourself to find out. The illustrations themselves had us smirking. We took the time to really look at the pages and discovered small details that really added to the enjoyment of the story. We read this story several times which is an indicator that this book is a keeper. Enjoy reading Summer 2023! originally posted at long and short reviews
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Reviewed in the United States on July 13, 2023
M
Meghan Downs
San Leandro, US
★★★★★ 5
So much fun!
Format: Hardcover
A hilarious book that keeps pages turning to see which wild pet will be next
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Reviewed in the United States on February 26, 2023
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Gabby
Belleville, US
★★★★★ 5
As good As It Get
Format: Kindle
I read the second book in this cottage series about smart town/village life in the Cotswolds before this first one. Both are so very very good and touching. Can't wait to read the third one in this series. These sapphic HEA romances are as good as it gets.
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Reviewed in the United States on November 11, 2025
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HM Kidd
Carnegie, US
★★★★★ 4
Sweet, Escapist, and Good Characters
Format: Kindle
I enjoyed the setting of this book. I love a book with a good sense of place. The chemistry between the characters was good and built through the story, I thought the book could have benefited from heavier editing. I appreciated the good dialogue, but it seemed unnecessarily copious at times, putting a drag on the forward motion of the story. Worth my time - absolutely.
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Reviewed in the United States on September 18, 2019

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