SKU: 90023144198

Human S100A10 (Protein S100-A10) ELISA Kit

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Description

Human S100A10 (Protein S100-A10) ELISA KitThe Human S100A10 (Protein S100 A10) ELISA Kit measures S100A10 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti S100A10 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated

The Human S100A10 (Protein S100-A10) ELISA Kit measures S100A10 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti S100A10 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with S100A10 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of S100A10 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 90023144198

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4.1 ★★★★★
Based on 23 reviews
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Verified Purchase
Jay
Belleville, US
★★★★★ 5
Our perfect chew toy for a relentless chewer!
Size: 2 Count (Pack of 1), Style: Pack of 2
We have looked high and low for a ball that our Australian Shepherd couldn't destroy. Read on to find out why we are sticking with this one. We've used other Chuckit! products in the past to mixed degrees of success. We absolutely love the ring fetch toy, though the dog can destroy those rings in a couple days. We tried other "nearly indestructible" balls only to have to throw them away within a couple days because our dog had destroyed them and left small pieces around the house for the baby to find. We have been COMPLETELY satisfied by these balls. They fit with the Chuckit! stick, which is a great bonus, but the most important thing is the dog hasn't been able to get pieces torn out of them. His orange one is his "indoor toy" and he's been going to town on it for weeks without any indication of wear. We periodically inspect it and are always floored to see that it's generally in the same condition as when we gave it to him. The orange color did fade a little when he left it outside for a few days in the sun, but considering how well it works otherwise, this didn't concern us at all. The "whistling" aspect of this ball is... well, an interesting gimmick but I wouldn't buy it if that's what you're interested in. Sometimes it makes noise in flight, other times it doesn't. We don't care. He chews it until he's tired of it (which is usually hours) and it squeaks and squorks on his teeth which keeps him happy as a clam. We honestly didn't think we'd find a ball that could keep up with his chewing, but this one has been phenomenal. We cannot recommend it enough!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 30, 2021
R
Verified Purchase
RR
Lexington, US
★★★★★ 5
So Far So Good
I adopted a young adult dog less than 2 weeks ago. She had spent over 12 hours a day in a crate and has excessive energy and anxiety built up. She is a strong chewer. So far, out of 6 toys, this is the only one still in one piece. It appears sturdy and extra bouncy. So much better than regular tennis balls.
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Reviewed in the United States on January 17, 2024
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Verified Purchase
Lisa B
Omaha, US
★★★★★ 5
My dogs favorite!
My dogs love these! They will play all day if you'd let them, these balls are their favorites. I use the chuck-it handle and they get tons of exercise in the big yard!
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Reviewed in the United States on April 22, 2026
T
Verified Purchase
Theredirishman
West Palm Beach, US
★★★★★ 5
The Dog LOVES IT!
I bought this for my ex-wife's Pitbull. He is an aggressive chewer and usually destroys any toy I buy him. But not this one! The ball is small enough and pliable that it gives when he chomps on it. He loves this ball and seems to be his favorite toy. Thank you for making a product that he likes and can't destroy!
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Reviewed in the United States on December 30, 2024
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Verified Purchase
Sean
Omaha, US
★★★★★ 5
My Golden Retriever’s favorite
We’ve owned lots of toys and balls. This specific version (in blue) has been her absolute favorite. She’s about 72lbs and chews on this thing regularly with no visible damage. We actually own about 6 of them because they have a tendency to go missing underneath furniture and in the yard. We have two of the orange colored ones but she doesn’t like those nearly as much which I assume is because the blue is easier to see in the grass. Other than that…They bounce really well and also whistle a little when you throw them hard enough. I hope they never stop making these.
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Reviewed in the United States on October 6, 2024

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