SKU: 89199326546

Human EIF3A (Eukaryotic translation initiation factor 3 subunit A) ELISA Kit

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Description

Human EIF3A (Eukaryotic translation initiation factor 3 subunit A) ELISA KitThe Human EIF3A (Eukaryotic translation initiation factor 3 subunit A) ELISA Kit measures EIF3A in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti EIF3A antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound

The Human EIF3A (Eukaryotic translation initiation factor 3 subunit A) ELISA Kit measures EIF3A in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti EIF3A antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with EIF3A bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of EIF3A in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 89199326546

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4.0 ★★★★★
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Verified Purchase
Andrea
New York, US
★★★★★ 5
Ball
Color: Aqua
Love it. My golden doesn’t like when it runs by itself but shepherds loves it
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on October 25, 2025
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Verified Purchase
Heston Killip
Waukegan, US
★★★★★ 3
Not all it’s cracked up to be.
Color: Aqua
Doesn’t really bounce all that much. Doesn’t really stay on either. My dobe could care less about it.
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Reviewed in the United States on January 5, 2026
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KD
New York, US
★★★★★ 5
Super entertaining toy if your dog likes balls and hunting
Color: Aqua
Love this! We got a similar ball for our dog about 6 months ago and she has been obsessed! We have 2 dogs, one hasa zero interest whatsoever in this toy (as expected) and the other will bark at it while its sitting on the charger so she can get it. Dogs, amirite? lol anyway, the issue we had with the previous one was that it was so loud on our wood floors because it was a solid plastic. The Cheerble ball has made a huge difference in sound for us! The ball itself is very quiet. When it rolls or bounces on something other than the floor you can barely hear it. With the previous ball we had, we couldn't be on the phone or watching a movie, etc if our dog was playing with the ball because it was so dang loud but this solves our problems! Its made of a hard silicone like material (thing a Nerf ball or something) so it remains quiet. The battery lasts as listed and its easy to charge. It took some time to figure out which setting our dog liked the best but after that it was smooth sailing. My only only negative to this is that the power on/off switch is inside so you have to twist it open to turn it off and on. The ball will naturally get some saliva on it so its kinda gross to put your hands all over it once its been played with and I'd prefer a button on the outside but understand why that isn't possible with this design. This ball is perfect if your dog is ball obsessed like ours. Shes a pitt/black lab/aussie mix so ball and hunting/chasing lizards are her favorite past times. If your dog prefers ripping toys apart to kill the squeaker (our other dog) or chewing bones, they may not take to this. Ball is life at our house and this ball is at the top of our pups list, there isn't a day that passes that she doesn't start her daily routine of seeking this out and begging us to turn it on. Highly recommend for the right pup!
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Reviewed in the United States on August 6, 2025
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Donna M.
Lexington, US
★★★★★ 5
Great toy
Color: Blue
Great toy, too bad my Shih Tzu is afraid of it. I love the way the movement is subtle and random.
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Reviewed in the United States on May 21, 2026
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Amazon Customer
Los Angeles, US
★★★★★ 5
Best toy for dogs
Color: Blue
My dog plays with this for hours!
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Reviewed in the United States on May 14, 2026

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