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Description
Human TLR4 (Toll-like Receptor 4) ELISA KitThe Human (TLR4) Toll like Receptor 4 ELISA Kit measures Toll Like Receptor 4 in samples. The plate has been pre coated with Human TLR4 antibody. TLR4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human TLR4 Antibody is added and binds to TLR4 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated TLR4 antibody. After incubation unbound Streptavidin HRP is washed away during a
The Human (TLR4) Toll-like Receptor 4 ELISA Kit measures Toll-Like Receptor 4 in samples. The plate has been pre-coated with Human TLR4 antibody. TLR4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human TLR4 Antibody is added and binds to TLR4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TLR4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human TLR4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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