Rat PACAP-27 (Pituitary Adenylate Cyclase Activating Polypeptide 27) ELISA Kit

Rat PACAP-27 (Pituitary Adenylate Cyclase Activating Polypeptide 27) ELISA KitThe Rat PACAP 27 (Pituitary Adenylate Cyclase Activating Polypeptide 27) ELISA Kit measures PACAP 27 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with PACAP 27. PACAP 27 in the sample or standard competes with a fixed amount of biotin labeled PACAP 27 for sites on a pre coated antibody

The Rat PACAP-27 (Pituitary Adenylate Cyclase Activating Polypeptide 27) ELISA Kit measures PACAP-27 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with PACAP-27. PACAP-27 in the sample or standard competes with a fixed amount of biotin-labeled PACAP-27 for sites on a pre-coated antibody specific to PACAP-27. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), biotin specifically binds with streptavidin to form immune complexes. Wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of PACAP-27 in the sample. The concentration of the target substance is inversely proportional to the OD450 value.