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Description
Human UCP1 (Mitochondrial Brown Fat Uncoupling Protein 1) ELISA KitThe Human (UCP1) Mitochondrial Brown Fat Uncoupling Protein 1 ELISA Kit measures Mitochondrial Brown Fat Uncoupling Protein 1 in samples. The plate has been pre coated with Human UCP1 antibody. UCP1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human UCP1 Antibody is added and binds to UCP1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated UCP1 antibody. After incubation
The Human (UCP1) Mitochondrial Brown Fat Uncoupling Protein 1 ELISA Kit measures Mitochondrial Brown Fat Uncoupling Protein 1 in samples. The plate has been pre-coated with Human UCP1 antibody. UCP1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human UCP1 Antibody is added and binds to UCP1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated UCP1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human UCP1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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