Mouse IgE (Immunoglobulin E) ELISA Kit

Mouse IgE (Immunoglobulin E) ELISA KitThis Mouse IgE (Immunoglobulin E) ELISA Kit uses the sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Mouse IgE. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IgE and AvidinHorseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and

This Mouse IgE (Immunoglobulin E) ELISA Kit uses the sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IgE. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IgE and AvidinHorseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IgE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is
terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 +/- 2 nm. The OD value is proportional to the concentration of Mouse IgE. You can calculate the concentration of Mouse IgE in the samples by comparing the OD of the samples to the standard curve.