SKU: 79067508943

Equine IL-10 (Interleukin 10) ELISA Kit

Sale price$263.25 Regular price$292.50
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Ships within 48 hours · Estimated delivery Sep 18 - Sep 23

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Description

Equine IL-10 (Interleukin 10) ELISA KitThe Equine IL 10 (Interleukin 10) ELISA Kit measures IL 10 in Equine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL 10 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection

The Equine IL-10 (Interleukin 10) ELISA Kit measures IL-10 in Equine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL-10 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with IL-10 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of IL-10 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 79067508943

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