TICAM1 (Toll Like Receptor Adaptor Molecule 1) ELISA Kit (Small Sample)

TICAM1 (Toll Like Receptor Adaptor Molecule 1) ELISA Kit (Small Sample)The TICAM1 (Toll Like Receptor Adaptor Molecule 1) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human TICAM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human TICAM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB

The TICAM1 (Toll Like Receptor Adaptor Molecule 1) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TICAM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TICAM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TICAM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TICAM1 in the samples is then determined by comparing the OD of the samples to the standard curve.