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Description
Human SEMA4G (Semaphorin 4G) ELISA KitThe Human (SEMA4G) Semaphorin 4G ELISA Kit measures Semaphorin 4G in samples. The plate has been pre coated with Human SEMA4G antibody. SEMA4G present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SEMA4G Antibody is added and binds to SEMA4G in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SEMA4G antibody. After incubation unbound Streptavidin HRP is washed away during a
The Human (SEMA4G) Semaphorin 4G ELISA Kit measures Semaphorin 4G in samples. The plate has been pre-coated with Human SEMA4G antibody. SEMA4G present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SEMA4G Antibody is added and binds to SEMA4G in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SEMA4G antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human SEMA4G. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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