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Description
MDA (Malondialdehyde) ELISA KitThe MDA (Malondialdehyde) ELISA immunoassay kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with MDA. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin conjugated antibody specific to MDA. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme substrate
The MDA (Malondialdehyde) ELISA immunoassay kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with MDA. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to MDA. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MDA in the samples is then determined by comparing the OD of the samples to the standard curve.
Shipping Notes
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Exchange/Return Notes
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