SKU: 71419052674

Human NEF3 (Neurofilament 3) ELISA Kit

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Description

Human NEF3 (Neurofilament 3) ELISA KitThe Human NEF3 (Neurofilament 3) ELISA Kit measures NEF3 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti NEF3 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection

The Human NEF3 (Neurofilament 3) ELISA Kit measures NEF3 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti NEF3 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with NEF3 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of NEF3 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 71419052674

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Amazon Customer
New York, US
★★★★★ 5
An international romance challenge that will keep you on edge
Format: Kindle
I I was intrigued with the complexity of just trying to live together with someone from a different country. This book clearly demonstrates the obstacles and I kept rooting for love to prevail.
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Reviewed in the United States on March 5, 2026
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Sally
Battle Creek, US
★★★★★ 5
A Must Read For Senior Sapphic Women
Format: Paperback
This is one of the best books for older sapphic women that I've ever read. It is so refreshing to not only have books for gay women but even better to have them for the "gray hair" crowd. Grayhall is a wonderful writer that keeps you spellbound. Three cheers for her.
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Reviewed in the United States on March 24, 2026
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J. Hatch
Pawtucket, US
★★★★★ 5
Kudos for A Place for Us!
Format: Kindle
A contemporary love story that doesn’t disappoint . We cheer on Jo and Lauren as they tackle the ups and downs, challenges and risks that come with any love affair. But this one is a special test to the two women’s commitment to each other, as society’s laws and opinions are the biggest hurdles they must face. Enmeshed in their current dysfunctional relationships with other people, Jo and Lauren are determined to have a second go at the true love they both had years ago, but that slipped away. Grayhall does a great job at ramping it up in the final chapters for an ending that truly delivers.
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Reviewed in the United States on June 8, 2025
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David Charles Logan, MD, MPH
West Palm Beach, US
★★★★★ 4
An inspiring and engaging tale.
Format: Kindle
A Place for Us is a heart-felt story of love, redemption and second chances.
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Reviewed in the United States on August 12, 2025
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Amazon Customer
Whiting, US
★★★★★ 5
A Place for Us
Format: Kindle
Oh! 15 gold stars for this one! This book is so poignant and so very brave and authentic! This book is a permission for us to choose what we will and will not accept in our relationships. This book is more than hope, this book is determination! Pure driven determination to not settle, but to go to the mat to have our life the way we want we want it. I cannot over recommend this book. Thank you, Patricia Grayhall! Keep writing!
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Reviewed in the United States on June 8, 2025

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