SKU: 62783511084

Mouse IRF5 (Interferon regulatory factor 5) QuickTest ELISA Kit

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Description

Mouse IRF5 (Interferon regulatory factor 5) QuickTest ELISA KitThe Mouse IRF5 (Interferon regulatory factor 5) QuickTest ELISA Kit measures Irf5 in Mouse Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and pilot

The Mouse IRF5 (Interferon regulatory factor 5) QuickTest ELISA Kit measures Irf5 in Mouse Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap/Det Ab working solution into each well, then add the standards and pilot samples into individual wells. If the sample contains Irf5, a capture antibody-Irf5-biotin-detection antibody complex was formed. After incubation, unbound conjugates were removed by wash buffer. HRP-Streptavidin was added. After washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of Irf5 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 62783511084

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Ryan
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Exactly the same as the free PDF I downloaded online for my student, only this costs $26 plus tax.
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This helped a lot for us to prepare for the SLATE exam.
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Reviewed in the United States on August 23, 2016

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