SKU: 6147623730

Rat C5a (Complement Component 5a) ELISA Kit

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Ships within 48 hours · Estimated delivery Aug 7 - Aug 12

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Description

Rat C5a (Complement Component 5a) ELISA KitThe Rat C5a (Complement Component 5a) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Rat C5a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat C5a and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate

The Rat C5a (Complement Component 5a) ELISA immunoassay kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat C5a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat C5a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat C5a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat C5a. You can calculate the concentration of Rat C5a in the samples by comparing the OD of the samples to the standard curve.

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SKU: 6147623730

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4.4 ★★★★★
Based on 19 reviews
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Verified Purchase
waragon
Birmingham, US
★★★★★ 4
Our pup loves the cronch cronch!
Style: Ball, Size: Medium (Pack of 1)
I bought our 15 month old Coton several Chuck-It toys on an Amazon deal. He is incredibly playful, mischievous, and ball/toy obsessed, especially for anything that makes noise. He can also be very destructive so I love how durable most of their products are - they’re among our longest lasting toys and worth every penny. We have yet to take them to the dog park, but they’re a perfect size for sharing in play time. And they are keeping him busy at home - that’s a good thing. He loves investigating the crunchy-cronchy-crinkly sound which is a nice change from squeakers. It can withstand his destructive chewing well-being, gentle on his little teeth. I love that. Chuck-It is thinking outside the box with a variety of balls to keep dogs engaged! I do worry about the crinkle plastic inside because it is accessible because of the air holes and it feels a little sharp. So this will have to be an at home toy so we can monitor his play.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 28, 2026
M
Verified Purchase
Mystical Lady
San Leandro, US
★★★★★ 5
Great products all around
Style: Ball, Size: Medium (Pack of 2)
Any of these balls are great! My dogs love all of them. The squeaky ones are their favorite!
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Reviewed in the United States on March 12, 2026
R
Verified Purchase
Raquel
Battle Creek, US
★★★★★ 5
My dog loves it!
Style: Ball, Size: Medium (Pack of 1)
This crunch ball was a Christmas gift for my 65lbs fur baby. It’s now mid April and he still loves it. He loves running after it and chomping down on it. It is still in good shape despite all the chomping it’s gotta . I highly recommend. He goes back to this toy agin and again! Great buy.
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Reviewed in the United States on April 15, 2026
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Verified Purchase
HalfTrac
Chelsea, US
★★★★★ 5
Our first babies favorite and longest lasting ball yet and he is fixing to be 7.
Style: Ball, Size: Medium (Pack of 2), Style: Ball, Size: Medium (Pack of 2)
Toughest ball we have found yet. Our doberman loves Chuckit! Balls especially the crunch one. He spreads all the balls and toys we have bought but these lasted the longest. The crunch and the glow in the dark are usually his pick but he has several different choices.
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Reviewed in the United States on May 24, 2026
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Verified Purchase
Lorraine Nathanson
Lowell, US
★★★★★ 5
Colorful, Sturdy & Great Value
Color: Large Set of 4
Purchased for our two puppies when they were 2 months old and weighed about 14 pounds so theses balls were a little big for them to hold in their mouths but that didn't stop them from trying. While they did like these balls they much preferred softer soccer balls, the later of which did not hold up well at all. I had hoped they would play with these balls more when they were older but that hasn't happened 5 months later. I guess it's no fun if you can't tear the toy apart. Colors are very bright and they do enjoy chasing after them as long as I am the one rolling them across the room for them. Maybe when they progress to batting things with their paws they will start to do this for themselves.
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Reviewed in the United States on May 18, 2025

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