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Description
Rat Orexin A ELISA KitThe Rat Orexin A ELISA Kit measures Orexin A in Rat samples. The plate has been pre coated with Rat HCRT antibody. HCRT present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat HCRT Antibody is added and binds to HCRT in the sample. Then Streptavidin HRP is added and binds to the Biotinylated HCRT antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate solution is
The Rat Orexin A ELISA Kit measures Orexin A in Rat samples. The plate has been pre-coated with Rat HCRT antibody. HCRT present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat HCRT Antibody is added and binds to HCRT in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HCRT antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat HCRT. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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