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Description
Human PARP1 (Poly [Adp-Ribose] Polymerase 1) ELISA KitThe Human (PARP1) Poly [Adp Ribose] Polymerase 1 ELISA Kit measures Poly [Adp Ribose] Polymerase 1 in samples. The plate has been pre coated with Human PARP1 antibody. PARP1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PARP1 Antibody is added and binds to PARP1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated PARP1 antibody. After incubation unbound Streptavidin HRP
The Human (PARP1) Poly [Adp-Ribose] Polymerase 1 ELISA Kit measures Poly [Adp-Ribose] Polymerase 1 in samples. The plate has been pre-coated with Human PARP1 antibody. PARP1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PARP1 Antibody is added and binds to PARP1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PARP1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human PARP1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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