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Description
Human ANPEP (Aminopeptidase N) ELISA KitThe Human (ANPEP) Aminopeptidase N ELISA Kit measures Aminopeptidase N in samples. The plate has been pre coated with Human ANPEP antibody. ANPEP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ANPEP Antibody is added and binds to ANPEP in the sample. Then Streptavidin HRP is added and binds to the Biotinylated ANPEP antibody. After incubation unbound Streptavidin HRP is washed away during a
The Human (ANPEP) Aminopeptidase N ELISA Kit measures Aminopeptidase N in samples. The plate has been pre-coated with Human ANPEP antibody. ANPEP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ANPEP Antibody is added and binds to ANPEP in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ANPEP antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human ANPEP. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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