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Description
Mouse SEMA7A (Semaphorin-7A) ELISA KitThe Mouse (SEMA7A) Semaphorin 7A ELISA Kit measures Semaphorin 7A in Mouse samples. The plate has been pre coated with Mouse SEMA7A antibody. SEMA7A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse SEMA7A Antibody is added and binds to SEMA7A in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SEMA7A antibody. After incubation unbound Streptavidin HRP is washed away during
The Mouse (SEMA7A) Semaphorin-7A ELISA Kit measures Semaphorin-7A in Mouse samples. The plate has been pre-coated with Mouse SEMA7A antibody. SEMA7A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse SEMA7A Antibody is added and binds to SEMA7A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SEMA7A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse SEMA7A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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