SKU: 49449864457

Porcine GHRL (Ghrelin) ELISA Kit

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Description

Porcine GHRL (Ghrelin) ELISA KitThe Porcine GHRL (Ghrelin) ELISA Kit measures GHRL in Porcine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with GHRL. GHRL in the sample or standard competes with a fixed amount of biotin labeled GHRL for sites on a pre coated antibody specific to GHRL. Free components are washed off. After adding

The Porcine GHRL (Ghrelin) ELISA Kit measures GHRL in Porcine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with GHRL. GHRL in the sample or standard competes with a fixed amount of biotin-labeled GHRL for sites on a pre-coated antibody specific to GHRL. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), biotin specifically binds with streptavidin to form immune complexes. Wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of GHRL in the sample. The concentration of the target substance is inversely proportional to the OD450 value.

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SKU: 49449864457

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Batya Levine
West Palm Beach, US
★★★★★ 4
Adorable
Format: Paperback
Thank you Knopf Books for Young Readers and NetGalley for the advanced electronic review copy of this adorable book. This gorgeously illustrated story is about friendship and working together to solve problems. Can’t wait to share it with my students!
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Reviewed in the United States on April 2, 2023
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Anna A
New York, US
★★★★★ 5
fun, diversity filled, read
Format: Kindle
I think this was a great fantasy book with lots of magic about friendship, courage, and finding your identity, whatever that may be. I liked how even if demons seemed bad at first, they are just different. Everyone is.
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Lai H.
Draper, US
★★★★★ 5
So engaging. Couldn't put it down.
Format: Paperback
I read this book in one sitting. It's thrilling from the beginning to the end. Beautiful art, wonderful storytelling and just a wonder to read.
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Reviewed in the United States on March 27, 2023
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BC
Omaha, US
★★★★★ 5
Great book
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My granddaughter loves this book. I hope there are more to come.
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Reviewed in the United States on September 2, 2023
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ruyu wang
Houston, US
★★★★★ 5
Great read!
Format: Kindle
The characters are relateable, storyline is heartwarming and I love the artwork!! Can't wait for the next chap! Good job!
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Reviewed in the United States on March 22, 2023

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