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Description
Rat WNT5A (Protein Wnt-5A) ELISA KitThe Rat (WNT5A) Protein Wnt 5A ELISA Kit measures Protein Wnt 5a in Rat samples. The plate has been pre coated with Rat WNT5A antibody. WNT5A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat WNT5A Antibody is added and binds to WNT5A in the sample. Then Streptavidin HRP is added and binds to the Biotinylated WNT5A antibody. After incubation unbound Streptavidin HRP is washed away during a washing
The Rat (WNT5A) Protein Wnt-5A ELISA Kit measures Protein Wnt-5a in Rat samples. The plate has been pre-coated with Rat WNT5A antibody. WNT5A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat WNT5A Antibody is added and binds to WNT5A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated WNT5A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat WNT5A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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