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Description
Rat AP13 (Apelin 13) ELISA KitThe Rat AP13 (Apelin 13) ELISA immunoassay kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with Rat AP13. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin conjugated antibody specific to Rat AP13. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme
The Rat AP13 (Apelin 13) ELISA immunoassay kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat AP13. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to Rat AP13. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat AP13 in the samples is then determined by comparing the OD of the samples to the standard curve.
Shipping Notes
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Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
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