EasyStep Human TRF (Transferrin) ELISA Kit

EasyStep Human TRF (Transferrin) ELISA KitEach EasyStep ELISA kit comes with two extra strips of coated wells to run for standard curves ensuring you have more wells for your samples. The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human TRF, and the Human TRF standard plate wells that pre coated using protein related techniques are provided separately. Standard Sample Diluent

Each EasyStep ELISA kit comes with two extra strips of coated wells to run for standard curves ensuring you have more wells for your samples. The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TRF, and the Human TRF standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human TRF. After TMB substrate solution is added, only those wells that contain Human TRF and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TRF in the samples is then determined by comparing the OD of the samples to the standard curve.