SKU: 39602038698

Mouse SHH (Sonic Hedgehog Homolog) ELISA Kit (Small Sample)

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Description

Mouse SHH (Sonic Hedgehog Homolog) ELISA Kit (Small Sample)The Mouse SHH (Sonic Hedgehog Homolog) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Mouse SHH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Mouse SHH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is

The Mouse SHH (Sonic Hedgehog Homolog) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SHH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SHH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SHH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SHH in the samples is then determined by comparing the OD of the samples to the standard curve.

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SKU: 39602038698

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4.8 ★★★★★
Based on 8 reviews
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Judith Jane
Lowell, US
★★★★★ 5
A classic reclaimed!
This classic Little Golden Book was my first "favorite book" as a little girl of four years who adored her daddy. Knowing full well that copy didn't survive the 60 plus years that have passed, I was elated to find it still in print. I found I still knew many pages by heart! My goal is to read it unendingly to a grandson who adores his daddy. Though the pictures are dated, and some of the occupations don't exist anymore, the love and respect a hard-working family man deserves is still well communicated--and the love of a child for it's father is still lovingly affirmed. I'm delighted with my purchase!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 11, 2019
S
Verified Purchase
Susan B.
Lexington, US
★★★★★ 5
A childhood favorite
Format: Hardcover
I used to read this book over and over. I bought it for someone recently and re-read it with many tears. It's a real Golden classic! It's wholesome, cute, funny, and familial. Kids AND adults will love reading it. Plus, it transports you back to the 50s!
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Reviewed in the United States on February 12, 2013
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Verified Purchase
TheMrs.R
Draper, US
★★★★★ 4
Just fine
Format: Hardcover
This was a good book. My husbands job doesn't really fit into any of the categories, it's very basic normal man jobs, so any job out of the ordinary won't be present in the book. Still a cute book with a good ending :)
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Reviewed in the United States on November 18, 2015
R
Verified Purchase
Rache
Belleville, US
★★★★★ 5
Love kitties
Format: Hardcover
Such a sweet, nostalgic story! Katie the Kitten is adorable, with charming illustrations and a gentle tale kids love. A timeless Little Golden Book that’s just as lovely as I remembered.
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Reviewed in the United States on June 24, 2025
S
Verified Purchase
SJ Latshaw
Louisville, US
★★★★★ 5
I've wanted to find this book for years!
Format: Hardcover
This is without a doubt one of my favorite books from childhood. I still have the old copy that I grew up with--but over the years, it has lost its cover and one or two pages. Not too bad considering that it was 40+ years old. I have found a few copies online and bought one that was a Little Silver (not Golden) Book, but the artwork was completely different. I was also hesitant to pay for a used copy because "good condition" means so many different things to different people. So I would check for this book every time I went to a used bookstore or garage sale. My most recent online search brought the exciting news that it was being reprinted WITH the original artwork, so I got on a waiting list right away!!! I couldn't be happier. Thank you so much for bringing back a friend from my childhood. :)
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Reviewed in the United States on September 12, 2018

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