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Description
Human NUCB1 (Nucleobindin-1) ELISA KitThe Human (NUCB1) Nucleobindin 1 ELISA Kit measures Nucleobindin 1 in samples. The plate has been pre coated with Human NUCB1 antibody. NUCB1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human NUCB1 Antibody is added and binds to NUCB1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated NUCB1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing
The Human (NUCB1) Nucleobindin-1 ELISA Kit measures Nucleobindin-1 in samples. The plate has been pre-coated with Human NUCB1 antibody. NUCB1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human NUCB1 Antibody is added and binds to NUCB1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NUCB1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human NUCB1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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