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Description
Rat PC (Pyruvate Carboxylase, Mitochondrial) ELISA KitThe Rat (PC) Pyruvate Carboxylase, Mitochondrial ELISA Kit measures Pyruvate carboxylase, mitochondrial in Rat samples. The plate has been pre coated with Rat PC antibody. PC present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat PC Antibody is added and binds to PC in the sample. Then Streptavidin HRP is added and binds to the Biotinylated PC antibody. After incubation unbound Streptavidin HRP is washed
The Rat (PC) Pyruvate Carboxylase, Mitochondrial ELISA Kit measures Pyruvate carboxylase, mitochondrial in Rat samples. The plate has been pre-coated with Rat PC antibody. PC present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat PC Antibody is added and binds to PC in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PC antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat PC. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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