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Description
Rat GAD1 (Glutamate Decarboxylase 1) ELISA KitThe Rat (GAD1) Glutamate Decarboxylase 1 ELISA Kit measures Glutamate decarboxylase 1 in Rat samples. The plate has been pre coated with Rat GAD1 antibody. GAD1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat GAD1 Antibody is added and binds to GAD1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated GAD1 antibody. After incubation unbound Streptavidin HRP is washed away
The Rat (GAD1) Glutamate Decarboxylase 1 ELISA Kit measures Glutamate decarboxylase 1 in Rat samples. The plate has been pre-coated with Rat GAD1 antibody. GAD1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat GAD1 Antibody is added and binds to GAD1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GAD1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat GAD1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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